| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4306964 | B | Inhibition of wild-type human full length NNMT expressed in Escherichia coli BL21(DE3) cells assessed as reduction in 1-methyl-nicotinamide formation pre-incubated for 10 mins followed by AdoMet and nicotinamide addition measured after 30 mins by UHP-HILIC/Q-TOF-MS analysis | Homo sapiens | 29 | cell-based format | Scientific Literature | ||
| 2. | ALA4306965 | B | Inhibition of wild-type human full length NNMT expressed in Escherichia coli BL21(DE3) cells assessed as reduction in 1-methyl-nicotinamide formation at 250 uM pre-incubated for 10 mins followed by AdoMet and nicotinamide addition measured after 30 mins by UHP-HILIC/Q-TOF-MS analysis relative to control | Homo sapiens | 11 | cell-based format | Scientific Literature | ||
| 3. | ALA4306974 | F | Antiproliferative activity against human HSC2 cells assessed as reduction in cell viability at 1 to 100 uM measured up to 72 hrs by MTT assay | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
| 4. | ALA4306975 | B | Binding affinity to wild-type human full length NNMT expressed in Escherichia coli BL21(DE3)-codonplus-RIL cells by isothermal titration calorimetry | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
| 5. | ALA4416333 | B | Inhibition of N-terminal His6-tagged wild type human NNMT expressed in Escherichia coli NiCo21(DE3) assessed as reduction in 1-methylquinolinium level using quinoline as substrate in presence of SAM and measured every 27 seconds for 5.5 mins by fluorescence-based assay | Homo sapiens | 35 | assay format | Scientific Literature | ||
| 6. | ALA5098417 | B | Inhibition of recombinant NNMT (unknown origin) assessed as reduction in MNA concentration incubated for 10 mins by UHP-HILIC-MS analysis | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 7. | ALA5223203 | B | Inhibition of recombinant human NNMT assessed as formation of MNA using nicotinamide and AdoMet as substrate preincubated for 10 mins followed by substrate addition and measured after 30 mins by UHP-HILIC-MS analysis | Homo sapiens | 1 | single protein format | Scientific Literature |
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