# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4411113 | B | Inhibition of recombinant porcine brain PREP expressed in Escherichia coli assessed as formation of 7-amido-4-methylcoumarin using Suc-Gly-Pro-amido-4-methylcoumarin as substrate preincubated for 30 mins followed by substrate addition measured after 60 mins by fluorometric assay | Sus scrofa | 15 | assay format | Scientific Literature | ||
2. | ALA4411115 | B | Inhibition of mouse PREP in N2A cells co-transfected with luciferase assessed as remaining alpha-synuclein dimerization at 10 uM measured after 4 hrs by protein fragment complementation assay relative to control | Mus musculus | 7 | cell-based format | Scientific Literature | ||
3. | ALA4411116 | B | Inhibition of mouse PREP in N2A cells co-transfected with luciferase assessed as reduction in alpha-synuclein dimerization at 10 uM measured after 4 hrs by protein fragment complementation assay relative to control | Mus musculus | 12 | cell-based format | Scientific Literature | ||
4. | ALA4825560 | F | Induction of autophagy in human HEK293 cells expressing GFP-LC3B-RFP assessed as decrease in fluorescence at 10 uM after 24 hrs by multilabel counter method | Homo sapiens | 25 | cell-based format | Scientific Literature |