# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4362816 | Binding | Displacement of 5'FAM-LTFEHYWAQLTS from human recombinant N-terminal domain of MDM2 (1 to 118 residues) expressed in Escherichia coli BL21 (DE3) cells measured after 15 mins by fluorescence polarization assay | Homo sapiens | 58 | ALA4359887 | cell-based format | Scientific Literature | |
2. | ALA4362817 | Binding | Displacement of 5'FAM-LTFEHYWAQLTS from human recombinant MDMX (18 to 111 residues) expressed in Escherichia coli BL21 (DE3) cells measured after 30 mins by fluorescence polarization assay | Homo sapiens | 56 | ALA4359887 | cell-based format | Scientific Literature | |
3. | ALA4362833 | Binding | Binding affinity to 15N-labeled MDM2 (unknown origin) at p53 binding pocket assessed as weaker tight binding by measuring slow chemical exchange with signal splitting by 1H-15N HSQC NMR analysis | Homo sapiens | 12 | ALA4359887 | single protein format | Scientific Literature |