# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4325735 | ADME | Enzymatic stability assessed as alpha-chymotrypsin (unknown origin)-mediated compound hydrolysis at pH 7.8 at 0.1 mg/ml incubated for 24 hrs by HPLC-MS analysis | Homo sapiens | 6 | ALA4321882 | assay format | Scientific Literature | |
2. | ALA4325736 | Binding | Binding affinity to human MDM2 expressed in Escherichia coli expression system by using (FITC)-labeled p53 peptide based fluorescence polarization competitive assay | Homo sapiens | 14 | ALA4321882 | assay format | Scientific Literature | |
3. | ALA4325737 | Binding | Potency index, ratio of p53 Kd to test compound Kd for binding affinity to human MDM2 expressed in Escherichia coli expression system by using (FITC)-labeled p53 peptide based fluorescence polarization competitive assay | Homo sapiens | 2 | ALA4321882 | assay format | Scientific Literature | |
4. | ALA4325738 | Binding | Potency index, ratio of p53 IC50 to test compound IC50 for binding affinity to human MDM2 expressed in Escherichia coli expression system by using (FITC)-labeled p53 peptide based fluorescence polarization competitive assay | Homo sapiens | 1 | ALA4321882 | assay format | Scientific Literature | |
5. | ALA4325741 | Binding | Activation of p53 in human U2OS cells cotransfected with BP100-luc and CMV-lacZ gene assessed as luciferase gene expression at 30 uM incubated for 18 hrs by p53-dependent luciferase transcriptional activation assay | Homo sapiens | 4 | ALA4321882 | cell-based format | Scientific Literature | |
6. | ALA4325742 | Binding | Activation of p53 in human U2OS cells cotransfected with BP100-luc and CMV-lacZ gene assessed as lacZ gene expression at 30 uM incubated for 18 hrs by p53-dependent luciferase transcriptional activation assay | Homo sapiens | 2 | ALA4321882 | cell-based format | Scientific Literature | |
7. | ALA4325748 | Binding | Binding affinity to MDMX (unknown origin) by using (FITC)-labeled p53 peptide based fluorescence polarization competitive assay | Homo sapiens | 9 | ALA4321882 | single protein format | Scientific Literature | |
8. | ALA4325749 | ADME | Enzymatic stability assessed as pronase (unknown origin)-mediated compound hydrolysis at pH 7.8 at 0.1 mg/ml incubated for 24 hrs by HPLC-MS analysis | Homo sapiens | 6 | ALA4321882 | assay format | Scientific Literature |