# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4428139 | Binding | Activation of full-length GST-tagged recombinant human SIRT1 assessed as enzyme activity obtained without subtraction of intrinsic fluorescence using H3K9 as substrate at 50 uM after 30 mins by SIRTainty assay | Homo sapiens | 56 | ALA4428023 | single protein format | Scientific Literature | |
2. | ALA4428140 | Binding | Activation of full-length GST-tagged recombinant human SIRT1 assessed as enzyme activity obtained with subtraction of intrinsic fluorescence using H3K9 as substrate at 50 uM after 30 mins by SIRTainty assay | Homo sapiens | 51 | ALA4428023 | single protein format | Scientific Literature | |
3. | ALA5112707 | Binding | Activation of N-terminal SUMO-tagged human SIRT2 (43 to 356 residues) expressed in Escherichia coli assessed as fold activation at 100 uM using acetylated alpha tubulin-K25 as substrate in presence of NAD+ | Homo sapiens | 43 | ALA5108071 | assay format | Scientific Literature | |
4. | ALA5112710 | Binding | Activation of N-terminal his-tagged human SIRT5 (34 to 302 residues) expressed in Escherichia coli assessed as fold activation at 100 uM using CPS1-succ537 as substrate in presence of NAD+ | Homo sapiens | 13 | ALA5108071 | assay format | Scientific Literature | |
5. | ALA5112711 | Binding | Activation of N-terminal his-tagged human SIRT5 (34 to 302 residues) expressed in Escherichia coli assessed as fold activation at 10 uM using CPS1-succ537 as substrate in presence of NAD+ | Homo sapiens | 7 | ALA5108071 | assay format | Scientific Literature |