| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4353688 | B | Inhibition of N-terminal His-tagged recombinant human KDM4A (1 to 359 residues) expressed in Escherichia coli assessed as decrease in demethylation of substrate using peptide (ARTKQTARK(Me3)STGGKAPRKQLA-GGKbiotin) as substrate and 2OG as cosubstrate pre-incubated for 10 mins before substrate addition followed by centrifugation for 1 min and measured after 20 mins by AlphaScreen assay | Homo sapiens | 33 | ALA4350996 | assay format | Scientific Literature | |
| 2. | ALA4353689 | B | Inhibition of N-terminal GST-tagged recombinant human KDM4B (1 to 500 residues) expressed in baculovirus infected Sf9 insect cells assessed as decrease in demethylation of substrate using peptide (ARTKQTARK(Me3)STGGKAPRKQLA-GGKbiotin) as substrate and 2OG as cosubstrate pre-incubated for 10 mins before substrate addition followed by centrifugation for 1 min and measured after 20 mins by AlphaScreen assay | Homo sapiens | 33 | ALA4350996 | cell-based format | Scientific Literature | |
| 3. | ALA4353690 | B | Inhibition of KDM5B (unknown origin) assessed as decrease in demethylation of substrate using peptide (H3(1-21)K4-Me3-GGKBiotin) as substrate and 2OG as cosubstrate pre-incubated for 15 mins before substrate addition followed by centrifugation for 15 secs and measured after 20 mins by Alphascreen assay | Homo sapiens | 33 | ALA4350996 | single protein format | Scientific Literature | |
| 4. | ALA4353691 | B | Inhibition of KDM5C (unknown origin) assessed as decrease in demethylation of substrate using peptide (H3(1-21)K4-Me3-GGKBiotin) as substrate and 2OG as cosubstrate pre-incubated for 15 mins before substrate addition followed by centrifugation for 15 secs and measured after 20 mins by Alphascreen assay | Homo sapiens | 33 | ALA4350996 | single protein format | Scientific Literature | |
| 5. | ALA4353692 | B | Inhibition of KDM2A (unknown origin) by Alphascreen assay | Homo sapiens | 6 | ALA4350996 | single protein format | Scientific Literature | |
| 6. | ALA4353693 | B | Inhibition of KDM3A (unknown origin) by Alphascreen assay | Homo sapiens | 6 | ALA4350996 | single protein format | Scientific Literature | |
| 7. | ALA4353695 | B | Inhibition of KDM6B (unknown origin) at 100 uM by Alphascreen assay relative to control | Homo sapiens | 1 | ALA4350996 | single protein format | Scientific Literature | |
| 8. | ALA4353708 | B | Inhibition of full length human KDM4B assessed as decrease in demethylation of substrate using peptide (ARTKQTARK(Me3)STGGKAPRKQLA-GGKbiotin) as substrate and 0.25 uM 2OG as cosubstrate pre-incubated for 10 mins before substrate addition followed by centrifugation for 1 min and measured after 15 mins by AlphaScreen assay | Homo sapiens | 3 | ALA4350996 | single protein format | Scientific Literature | |
| 9. | ALA4353709 | B | Inhibition of full length human KDM4B assessed as decrease in demethylation of substrate using peptide (ARTKQTARK(Me3)STGGKAPRKQLA-GGKbiotin) as substrate and 0.5 uM 2OG as cosubstrate pre-incubated for 10 mins before substrate addition followed by centrifugation for 1 min and measured after 15 mins by AlphaScreen assay | Homo sapiens | 3 | ALA4350996 | single protein format | Scientific Literature | |
| 10. | ALA4353710 | B | Inhibition of full length human KDM4B assessed as decrease in demethylation of substrate using peptide (ARTKQTARK(Me3)STGGKAPRKQLA-GGKbiotin) as substrate and 1 uM 2OG as cosubstrate pre-incubated for 10 mins before substrate addition followed by centrifugation for 1 min and measured after 15 mins by AlphaScreen assay | Homo sapiens | 3 | ALA4350996 | single protein format | Scientific Literature | |
| 11. | ALA4353711 | B | Inhibition of full length human KDM4B assessed as decrease in demethylation of substrate using peptide (ARTKQTARK(Me3)STGGKAPRKQLA-GGKbiotin) as substrate and 2 uM 2OG as cosubstrate pre-incubated for 10 mins before substrate addition followed by centrifugation for 1 min and measured after 15 mins by AlphaScreen assay | Homo sapiens | 3 | ALA4350996 | single protein format | Scientific Literature | |
| 12. | ALA4353712 | B | Inhibition of full length human KDM4B assessed as decrease in demethylation of substrate using peptide (ARTKQTARK(Me3)STGGKAPRKQLA-GGKbiotin) as substrate and 4 uM 2OG as cosubstrate pre-incubated for 10 mins before substrate addition followed by centrifugation for 1 min and measured after 15 mins by AlphaScreen assay | Homo sapiens | 3 | ALA4350996 | single protein format | Scientific Literature | |
| 13. | ALA4353713 | B | Inhibition of full length human KDM4B assessed as decrease in demethylation of substrate using peptide (ARTKQTARK(Me3)STGGKAPRKQLA-GGKbiotin) as substrate and 8 uM 2OG as cosubstrate pre-incubated for 10 mins before substrate addition followed by centrifugation for 1 min and measured after 15 mins by AlphaScreen assay | Homo sapiens | 3 | ALA4350996 | single protein format | Scientific Literature | |
| 14. | ALA4353714 | B | Inhibition of full length human KDM4B assessed as decrease in demethylation of substrate using peptide (ARTKQTARK(Me3)STGGKAPRKQLA-GGKbiotin) as substrate and 16 uM 2OG as cosubstrate pre-incubated for 10 mins before substrate addition followed by centrifugation for 1 min and measured after 15 mins by AlphaScreen assay | Homo sapiens | 3 | ALA4350996 | single protein format | Scientific Literature | |
| 15. | ALA4353715 | B | Inhibition of full length human KDM4B assessed as decrease in demethylation of substrate using peptide (ARTKQTARK(Me3)STGGKAPRKQLA-GGKbiotin) as substrate and 50 uM 2OG as cosubstrate pre-incubated for 10 mins before substrate addition followed by centrifugation for 1 min and measured after 15 mins by AlphaScreen assay | Homo sapiens | 3 | ALA4350996 | single protein format | Scientific Literature | |
| 16. | ALA4353716 | B | Inhibition of full length human KDM4B assessed as decrease in demethylation of substrate using peptide (ARTKQTARK(Me3)STGGKAPRKQLA-GGKbiotin) as substrate and 100 uM 2OG as cosubstrate pre-incubated for 10 mins before substrate addition followed by centrifugation for 1 min and measured after 15 mins by AlphaScreen assay | Homo sapiens | 3 | ALA4350996 | single protein format | Scientific Literature | |
| 17. | ALA4353717 | B | Inhibition of full length human KDM4B assessed as decrease in demethylation of substrate using peptide (ARTKQTARK(Me3)STGGKAPRKQLA-GGKbiotin) as substrate and 300 uM 2OG as cosubstrate pre-incubated for 10 mins before substrate addition followed by centrifugation for 1 min and measured after 15 mins by AlphaScreen assay | Homo sapiens | 3 | ALA4350996 | single protein format | Scientific Literature | |
| 18. | ALA4353718 | B | Inhibition of full length human KDM4B assessed as decrease in demethylation of substrate using peptide (ARTKQTARK(Me3)STGGKAPRKQLA-GGKbiotin) as substrate and 1000 uM 2OG as cosubstrate pre-incubated for 10 mins before substrate addition followed by centrifugation for 1 min and measured after 15 mins by AlphaScreen assay | Homo sapiens | 3 | ALA4350996 | single protein format | Scientific Literature | |
| 19. | ALA4353730 | B | Binding affinity to KDM4A (unknown origin) | Homo sapiens | 8 | ALA4350996 | single protein format | Scientific Literature | |
| 20. | ALA4353731 | B | Binding affinity to KDM5B (unknown origin) | Homo sapiens | 8 | ALA4350996 | single protein format | Scientific Literature |
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