# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4347273 | Binding | Agonist activity at human TRPA1 expressed in HEK293 cells assessed as increase in calcium influx at 30 uM by Fluo-4-AM dye based fluorescence assay relative to calcimycin | Homo sapiens | 20 | ALA4346663 | cell-based format | Scientific Literature | |
2. | ALA4347281 | Binding | Antagonist activity at human TRPA1 expressed in HEK293 cells assessed as increase in calcium influx at 3 uM incubated for 10 mins prior to CA challenge by Fluo-4-AM dye based fluorescence assay relative to calcimycin | Homo sapiens | 4 | ALA4346663 | cell-based format | Scientific Literature | |
3. | ALA4347282 | Binding | Agonist activity at human TRPM8 expressed in HEK293 cells assessed as increase in calcium influx at 3 uM by Fluo-4-AM dye based fluorescence assay relative to calcimycin | Homo sapiens | 3 | ALA4346663 | cell-based format | Scientific Literature | |
4. | ALA4347284 | Binding | Antagonist activity at human TRPM8 expressed in HEK293 cells assessed as increase in calcium influx at 30 uM incubated for 10 mins prior to WS5 challenge by Fluo-4-AM dye based fluorescence assay relative to WS5 | Homo sapiens | 1 | ALA4346663 | cell-based format | Scientific Literature | |
5. | ALA4347300 | Physicochemical | Dissociation constant, pKa of the compound at pH 7.4 | 35 | ALA4346663 | small-molecule physicochemical format | Scientific Literature | ||
6. | ALA4347309 | Binding | Agonist activity at human TRPA1 Cys261Ala mutant expressed in HEK293 cells assessed as Ca2+ influx at 30 uM by fluorescence assay | Homo sapiens | 9 | ALA4346663 | cell-based format | Scientific Literature | |
7. | ALA4347316 | Binding | Agonist activity at human TRPA1 Phe909Ala mutant expressed in HEK293 cells assessed as Ca2+ influx at 30 uM by fluorescence assay | Homo sapiens | 12 | ALA4346663 | cell-based format | Scientific Literature | |
8. | ALA4347321 | Binding | Agonist activity at human TRPA1 Phe944Ala mutant expressed in HEK293 cells assessed as Ca2+ influx at 30 uM by fluorescence assay | Homo sapiens | 9 | ALA4346663 | cell-based format | Scientific Literature |