# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4325325 | Binding | Inhibition of N-terminal GST-fused full-length human CDC7 (1 to 574 end residues)/DBF4 (1 to 674 residues) expressed in baculovirus expression system using MCM2 as substrate preincubated with enzyme for 10 mins prior to 1 uM ATP addition by HTRF transcreener ADP assay | Homo sapiens | 17 | ALA4321874 | assay format | Scientific Literature | |
2. | ALA4325326 | Binding | Inhibition of N-terminal GST-tagged full-length human CDK2 (1 to 298 residues)/Cyclin E (1 to 410 residues) expressed in baculovirus expression system using histone H1 as susbtrate after 90 mins by Kinase-Glo luminescence assay | Homo sapiens | 17 | ALA4321874 | assay format | Scientific Literature | |
3. | ALA4325327 | Binding | Inhibition of N-terminal GST-tagged human ROCK1 catalytic domain (1 to 477 residues) expressed in baculovirus expression system using biotin-STK-substrate 2 as substrate preincubated with enzyme for 5 mins followed by ATP addition and measured after 2 hrs by TR-FRET assay | Homo sapiens | 17 | ALA4321874 | assay format | Scientific Literature | |
4. | ALA4325328 | Binding | Selectivity index, ratio of IC50 for N-terminal GST-tagged full-length human CDK2 (1 to 298 residues)/Cyclin E (1 to 410 residues) expressed in baculovirus expression system to IC50 for N-terminal GST-fused full-length human CDC7 (1 to 574 end residues)/DBF4 (1 to 674 residues) expressed in baculovirus expression system | Homo sapiens | 17 | ALA4321874 | assay format | Scientific Literature | |
5. | ALA4325329 | Binding | Selectivity index, ratio of IC50 N-terminal GST-tagged human ROCK1 catalytic domain (1 to 477 residues) expressed in baculovirus expression system to IC50 for N-terminal GST-fused full-length human CDC7 (1 to 574 end residues)/DBF4 (1 to 674 residues) expressed in baculovirus expression system | Homo sapiens | 17 | ALA4321874 | assay format | Scientific Literature |