# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4362816 | Binding | Displacement of 5'FAM-LTFEHYWAQLTS from human recombinant N-terminal domain of MDM2 (1 to 118 residues) expressed in Escherichia coli BL21 (DE3) cells measured after 15 mins by fluorescence polarization assay | Homo sapiens | 58 | ALA4359887 | cell-based format | Scientific Literature | |
2. | ALA4362817 | Binding | Displacement of 5'FAM-LTFEHYWAQLTS from human recombinant MDMX (18 to 111 residues) expressed in Escherichia coli BL21 (DE3) cells measured after 30 mins by fluorescence polarization assay | Homo sapiens | 56 | ALA4359887 | cell-based format | Scientific Literature | |
3. | ALA4362818 | Binding | Inhibition of MDM2-p53 interaction in human U2OS cells expressing wild-type p53 assessed as upregulation of MDM2 expression at 20 to 50 uM measured after 24 hrs by Western blot analysis | Homo sapiens | 3 | ALA4359887 | cell-based format | Scientific Literature | |
4. | ALA4362819 | Binding | Inhibition of MDM2-p53 interaction in human SJSA1 cells expressing wild-type p53 assessed as upregulation of p21 expression at 20 to 50 uM measured after 24 hrs by Western blot analysis | Homo sapiens | 3 | ALA4359887 | cell-based format | Scientific Literature | |
5. | ALA4362820 | Binding | Inhibition of MDM2-p53 interaction in human SJSA1 cells expressing wild-type p53 assessed as upregulation of MDM2 expression at 20 to 50 uM measured after 24 hrs by Western blot analysis | Homo sapiens | 3 | ALA4359887 | cell-based format | Scientific Literature | |
6. | ALA4362821 | Binding | Inhibition of MDM2-p53 interaction in human U2OS cells expressing wild-type p53 assessed as upregulation of p21 expression at 20 to 50 uM measured after 24 hrs by Western blot analysis | Homo sapiens | 3 | ALA4359887 | cell-based format | Scientific Literature | |
7. | ALA4362822 | Functional | Anticancer activity against p53-deleted human Saos2 cells assessed as inhibition of cell viability measured after 5 days by MTT assay | Homo sapiens | 3 | ALA4359887 | cell-based format | Scientific Literature | |
8. | ALA4362823 | Functional | Anticancer activity against human U2OS cells expressing wild-type p53 assessed as inhibition of cell viability measured after 5 days by MTT assay | Homo sapiens | 3 | ALA4359887 | cell-based format | Scientific Literature |