Assay

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Assay

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	#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
	1.	ALA4409428	B	Inhibition of recombinant human C-terminal His8-tagged IDH1 R132C mutant expressed in Escherichia coli BL21 assessed as reduction in NADPH consumption using alpha-ketoglutarate as substrate preincubated for 60 mins followed by substrate addition and measured for 60 mins in presence of NADPH by diaphorase/resazurin-based fluorescence assay	Homo sapiens	26		assay format	Scientific Literature
	2.	ALA4409429	B	Inhibition of recombinant human C-terminal His8-tagged IDH1 R132H mutant expressed in Escherichia coli BL21 assessed as reduction in NADPH consumption using alpha-ketoglutarate as substrate preincubated for 60 mins followed by substrate addition measured for 60 mins in presence of NADPH by diaphorase/resazurin-based assay	Homo sapiens	26		assay format	Scientific Literature