#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4433999	A	Effect on IL-6 mRNA expression in TLR2-/- mouse BMDM cells at 0.1 to 1 uM by qRT-PCR analysis	Mus musculus	4	ALA4433228	cell-based format	Scientific Literature
2.	ALA4434000	F	Effect on IL-6 production in TLR2-/- mouse BMDM cells at 0.1 to 1 uM incubated for 24 hrs by ELISA	Mus musculus	7	ALA4433228	cell-based format	Scientific Literature
3.	ALA4434001	F	Effect on TNFalpha production in TLR2-/- mouse BMDM cells at 0.1 to 1 uM incubated for 24 hrs by ELISA	Mus musculus	3	ALA4433228	cell-based format	Scientific Literature
4.	ALA4434002	B	Agonist activity at TLR2 in mouse BV2 cells assessed as increase in IL-6 secretion at 1 uM incubated for 24 hrs in presence of NF-kappaB inhibitor triptolide by ELISA	Mus musculus	3	ALA4433228	cell-based format	Scientific Literature
5.	ALA4434003	B	Activation of TLR2 in in wild type mouse BMDM cells assessed as stimulation of IL-6 production at 0.1 to 1 uM incubated for 24 hrs by ELISA	Mus musculus	3	ALA4433228	cell-based format	Scientific Literature
6.	ALA4434004	A	Effect on IL-6 mRNA expression in TLR2-/- mouse BMDM cells at 0.1 to 1 uM by qRT-PCR analysis	Mus musculus	4	ALA4433228	cell-based format	Scientific Literature
7.	ALA4434005	B	Agonist activity at TLR2 in wild type mouse BMDM cells assessed as induction of NFkappaB pathway activation by measuring increase in IL-6 mRNA expression by qRT-PCR analysis	Mus musculus	4	ALA4433228	cell-based format	Scientific Literature
8.	ALA4434006	B	Agonist activity at TLR2 in mouse dendritic cells assessed as upregulation CD80 expression at 100 ng/ml by flow cytometry	Mus musculus	4	ALA4433228	cell-based format	Scientific Literature
9.	ALA4434013	B	Agonist activity at TLR2/1 in PMA-differentiated human THP-1 cells assessed as induction of IL-6 production at 2.5 uM incubated for 24 hrs after preincubation with anti-hTLR2-IGA for 2 hrs by ELISA	Homo sapiens	6	ALA4433228	cell-based format	Scientific Literature
10.	ALA4434014	B	Agonist activity at TLR2/1 in PMA-differentiated human THP-1 cells assessed as induction of IL-6 production at 2.5 uM incubated for 24 hrs after preincubation with anti-hTLR1-IGG for 2 hrs by ELISA	Homo sapiens	3	ALA4433228	cell-based format	Scientific Literature
11.	ALA4434015	B	Agonist activity at TLR2/6 in PMA-differentiated human THP-1 cells assessed as induction of IL-6 production at 2.5 uM incubated for 24 hrs after preincubation with anti-hTLR6-IGG for 2 hrs by ELISA	Homo sapiens	3	ALA4433228	cell-based format	Scientific Literature
12.	ALA4434019	A	Cytotoxicity against mouse BV2 cells assessed as cell viability up to 5 uM incubated for 24 hrs by CCK-8 assay	Mus musculus	4	ALA4433228	cell-based format	Scientific Literature
13.	ALA4434020	B	Agonist activity at TLR2 in mouse BV2 cells assessed as increase in IL-6 secretion at 2 uM incubated for 24 hrs by ELISA	Mus musculus	4	ALA4433228	cell-based format	Scientific Literature
14.	ALA4434022	B	Activation of TLR2 in in wild type mouse BMDM cells assessed as stimulation of IL-12 production at 0.1 to 1 uM incubated for 24 hrs by ELISA	Mus musculus	3	ALA4433228	cell-based format	Scientific Literature
15.	ALA4434023	B	Activation of TLR2 in in wild type mouse BMDM cells assessed as stimulation of TNFalpha production at 0.1 to 1 uM incubated for 24 hrs by ELISA	Mus musculus	3	ALA4433228	cell-based format	Scientific Literature
16.	ALA4434024	A	Effect on IkappaBalpha protein expression in mouse BV2 cells at 0.2 to 2 uM incubated for 12 hrs by Western blot analysis	Mus musculus	3	ALA4433228	cell-based format	Scientific Literature