#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4385196	B	Inhibition of calf thymus topoisomerase-1 assessed as reduction in cleavage complex formation by measuring decrease in nicked form DNA using supercoiled pBR322 DNA as substrate after 30 mins by agarose gel electrophoresis assay	Bos taurus	1	ALA4382271	single protein format	Scientific Literature
2.	ALA4385197	B	Inhibition of calf thymus topoisomerase-1 assessed as reduction in cleavage complex formation by measuring nicked form DNA level using supercoiled pBR322 DNA as substrate after 30 mins by agarose gel electrophoresis assay (Rvb = 47%)	Bos taurus	3	ALA4382271	single protein format	Scientific Literature
3.	ALA4385198	B	Inhibition of supercoiled pBR322 DNA binding to calf thymus topoisomerase-1 after 0.5 hrs by agarose gel electrophoresis assay	Bos taurus	2	ALA4382271	single protein format	Scientific Literature
4.	ALA4385199	B	Inhibition of calf thymus topoisomerase-1-mediated supercoiled pBR322 DNA unwinding at 25 uM preincubated with DNA for 10 mins followed by enzyme addition and measured after 30 mins by agarose gel electrophoresis assay	Bos taurus	3	ALA4382271	single protein format	Scientific Literature
5.	ALA4385200	B	Inhibition of calf thymus topoisomerase-1-mediated relaxed pBR322 DNA unwinding at 25 uM preincubated with DNA for 10 mins followed by enzyme addition and measured after 30 mins by agarose gel electrophoresis assay	Bos taurus	3	ALA4382271	single protein format	Scientific Literature