# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4322320 | B | Inhibition of biotinylated Bim peptide (81 to 106 residues) binding to His-tagged human Mcl-1 expressed in Escherichia coli BL21 (DE3) cells preincubated for 1 hr followed by biotinylated Bim peptide addition measured after 2 hrs by ELISA | Homo sapiens | 15 | ALA4321796 | cell-based format | Scientific Literature | |
2. | ALA4322321 | B | Inhibition of biotinylated Bim peptide (81 to 106 residues) binding to His-tagged human Bcl-2 expressed in Escherichia coli BL21 (DE3) cells preincubated for 1 hr followed by biotinylated Bim peptide addition measured after 2 hrs by ELISA | Homo sapiens | 15 | ALA4321796 | cell-based format | Scientific Literature | |
3. | ALA4322322 | B | Induction of CRBN-mediated Mcl-1 degradation in human HeLa cells assessed as relative abundance at 10 uM after 12 hrs by Western blot analysis (Rvb = 1 No_unit) | Homo sapiens | 14 | ALA4321796 | cell-based format | Scientific Literature | |
4. | ALA4322323 | B | Induction of CRBN-mediated Mcl-1 degradation in human HeLa cells assessed as relative abundance at 1 uM after 12 hrs by Western blot analysis (Rvb = 1 No_unit) | Homo sapiens | 12 | ALA4321796 | cell-based format | Scientific Literature | |
5. | ALA4322324 | B | Induction of CRBN-mediated Bcl-2 degradation in human HeLa cells assessed as relative abundance at 10 uM after 12 hrs by Western blot analysis (Rvb = 1 No_unit) | Homo sapiens | 14 | ALA4321796 | cell-based format | Scientific Literature | |
6. | ALA4322325 | B | Induction of CRBN-mediated Bcl-2 degradation in human HeLa cells assessed as relative abundance at 1 uM after 12 hrs by Western blot analysis (Rvb = 1 No_unit) | Homo sapiens | 12 | ALA4321796 | cell-based format | Scientific Literature | |
7. | ALA4322326 | B | Induction of CRBN-mediated Mcl-1 degradation in human HeLa cells at 10 uM after 12 hrs by Western blot analysis relative to control | Homo sapiens | 7 | ALA4321796 | cell-based format | Scientific Literature | |
8. | ALA4322327 | B | Induction of CRBN-mediated Bcl-2 degradation in human HeLa cells at 10 uM after 12 hrs by Western blot analysis relative to control | Homo sapiens | 7 | ALA4321796 | cell-based format | Scientific Literature | |
9. | ALA4322369 | B | Protac activity at CRBN/Mcl-1 in human HeLa cell lysates assessed as ternary complex formation by Western blot analysis based pull-down assay | Homo sapiens | 2 | ALA4321796 | subcellular format | Scientific Literature | |
10. | ALA4322370 | B | Protac activity at CRBN/Bcl-2 in human HeLa cell lysates assessed as ternary complex formation by Western blot analysis based pull-down assay | Homo sapiens | 2 | ALA4321796 | subcellular format | Scientific Literature |