# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4420660 | B | Inhibition of human LATS1 kinase domain (589 to 1130 amino acids) incubated for 30 mins by HTRF assay | Homo sapiens | 244 | single protein format | Patent Bioactivity Data | ||
2. | ALA4420661 | B | Inhibition of human LATS1 kinase domain (589 to 1130 amino acids) incubated for 3 hrs in presence of FAM-KKLRRTLSVA-COOH by fluorescence detection based biochemical caliper assay | Homo sapiens | 5 | single protein format | Patent Bioactivity Data | ||
3. | ALA4420662 | B | Inhibition of human LATS2 kinase domain (553 to 1088 amino acids) incubated for 3 hrs in presence of FAM-KKLRRTLSVA-COOH by fluorescence detection based biochemical caliper assay | Homo sapiens | 244 | single protein format | Patent Bioactivity Data | ||
4. | ALA4420664 | B | Inhibition of LATS in human HaCaT cells assessed as reduction in YAP phosphorylation level incubated for 2 hrs by HTRF assay | Homo sapiens | 244 | cell-based format | Patent Bioactivity Data | ||
5. | ALA4420665 | B | Inhibition of LATS in human HaCaT cells assessed as reduction in YAP nuclear translocation incubated for 24 hrs | Homo sapiens | 244 | cell-based format | Patent Bioactivity Data | ||
6. | ALA4420671 | B | Inhibition of LATS in human JHH5 cells assessed as reduction in YAP phosphorylation level incubated for 2 hrs by HTRF assay | Homo sapiens | 284 | cell-based format | Patent Bioactivity Data | ||
7. | ALA4420680 | B | Inhibition of LATS in human limbal epithelial cells assessed as increase in YAP level at 10 uM incubated for 24 hrs by immunostaining based assay | Homo sapiens | 2 | cell-based format | Patent Bioactivity Data | ||
8. | ALA4420681 | B | Inhibition of LATS in human limbal epithelial cells assessed as reduction in YAP phosphorylation level at 10 uM incubated for 1 hr by Western blot analysis | Homo sapiens | 2 | cell-based format | Patent Bioactivity Data | ||
9. | ALA4420682 | B | Inhibition of LATS in human limbal epithelial cells assessed as induction of limbal stem cell expansion by measuring p63alpha expression at 10 uM measured after 2 weeks by immunohistochemistry | Homo sapiens | 2 | cell-based format | Patent Bioactivity Data | ||
10. | ALA4420683 | B | Inhibition of LATS in human limbal epithelial cells assessed as induction of limbal stem cell expansion by measuring expansion factor ratio at 10 uM measured after 2 weeks by p63alpha expression detection based immunohistochemistry relative to control | Homo sapiens | 18 | cell-based format | Patent Bioactivity Data | ||
11. | ALA4420684 | B | Inhibition of LATS in human limbal epithelial cells assessed as induction of limbal stem cell expansion by measuring measuring ratio of expanded population of cells to population of seeded cells at 10 uM measured after 2 weeks by p63alpha expression detection based immunohistochemistry | Homo sapiens | 18 | cell-based format | Patent Bioactivity Data | ||
12. | ALA4420685 | B | Inhibition of LATS in human limbal epithelial cells assessed as induction of limbal stem cell expansion by measuring p63alpha expression at 10 uM measured after 2 weeks by immunohistochemistry relative to control | Homo sapiens | 18 | cell-based format | Patent Bioactivity Data | ||
13. | ALA4420686 | B | Inhibition of LATS in human limbal epithelial cells assessed as induction of limbal stem cell expansion marker expression by measuring deltaN-p63-alphabetagamma protein expression at 10 uM incubated for 8 to 10 days by immunohistochemistry | Homo sapiens | 2 | cell-based format | Patent Bioactivity Data | ||
14. | ALA4420687 | B | Inhibition of LATS in human limbal epithelial cells assessed as induction of limbal stem cell expansion marker expression by measuring ABCG2 protein expression at 10 uM incubated for 8 to 10 days by immunohistochemistry | Homo sapiens | 2 | cell-based format | Patent Bioactivity Data | ||
15. | ALA4420688 | B | Inhibition of LATS in human limbal epithelial cells assessed as induction of limbal stem cell expansion marker expression by measuring C/EBPdelta protein expression at 10 uM incubated for 8 to 10 days by immunohistochemistry | Homo sapiens | 2 | cell-based format | Patent Bioactivity Data | ||
16. | ALA4420689 | B | Inhibition of LATS in human limbal epithelial cells assessed as induction of cell differentiation into corneal epithelial cells by measuring keratin-12 expression at 10 uM incubated for 8 to 10 days followed by medium change to DMEM medium without serum and compound for further incubation for 4 to 8 days by confocal microscopy | Homo sapiens | 4 | cell-based format | Patent Bioactivity Data | ||
17. | ALA4420695 | B | Inhibition of LATS in human corneal endothelium cells assessed as increase in YAP level at 10 uM incubated for 24 hrs by immunohistochemistry | Homo sapiens | 2 | assay format | Patent Bioactivity Data | ||
18. | ALA4420696 | B | Inhibition of LATS in human corneal endothelium cells assessed as reduction in YAP phosphorylation at 10 uM incubated for 1 hr by Western blot analysis | Homo sapiens | 2 | assay format | Patent Bioactivity Data | ||
19. | ALA4420697 | B | Inhibition of LATS in human corneal endothelium cells assessed as induction of cell proliferation measured every 3 hrs for 10 days | Homo sapiens | 2 | assay format | Patent Bioactivity Data | ||
20. | ALA4420698 | B | Inhibition of LATS in human corneal endothelium cells assessed as induction of cell expansion by measuring ratio of expanded population of cells to population of seeded cells at 10 uM by Sytox orange dye based epifluorescence microscopy | Homo sapiens | 21 | assay format | Patent Bioactivity Data |