# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4334826 | Binding | Inhibition of human immunoproteasome beta 5i subunit using Suc-LLVY-AMC as substrate preincubated for 30 mins followed by substrate addition and measured for 120 mins by fluorescence based assay | Homo sapiens | 12 | ALA4334435 | single protein format | Scientific Literature | |
2. | ALA4334829 | Binding | Inhibition of human immunoproteasome beta 2i subunit at 10 uM using Boc-LRR-AMC as substrate preincubated for 30 mins followed by substrate addition measured for 120 mins by fluorescence based assay | Homo sapiens | 10 | ALA4334435 | single protein format | Scientific Literature | |
3. | ALA4334830 | Binding | Inhibition of human immunoproteasome beta 1i subunit at 10 uM using Ac-PAL-AMC as substrate preincubated for 30 mins followed by substrate addition measured for 120 mins by fluorescence based assay | Homo sapiens | 10 | ALA4334435 | single protein format | Scientific Literature | |
4. | ALA4334831 | ADME | Inhibition of human constitutive proteasome beta 5 subunit at 10 uM using Suc-LLVY-AMC as substrate preincubated for 30 mins followed by substrate addition measured for 120 mins by fluorescence based assay | Homo sapiens | 10 | ALA4334435 | single protein format | Scientific Literature | |
5. | ALA4334832 | ADME | Inhibition of human constitutive proteasome beta 2 subunit at 10 uM using Boc-LRR-AMC as substrate preincubated for 30 mins followed by substrate addition measured for 120 mins by fluorescence based assay | Homo sapiens | 10 | ALA4334435 | single protein format | Scientific Literature | |
6. | ALA4334833 | ADME | Inhibition of human constitutive proteasome beta 1 subunit at 10 uM using Z-LLE-AMC as substrate preincubated for 30 mins followed by substrate addition measured for 120 mins by fluorescence based assay | Homo sapiens | 10 | ALA4334435 | single protein format | Scientific Literature | |
7. | ALA4334834 | Binding | Inhibition of 20S immunoproteasome beta 5 chymotrypsin-like activity in human THP-1 cell lysates using Suc-LLVY-AMC as substrate preincubated for 30 mins followed by substrate addition and measured for 90 mins by fluorescence based assay | Homo sapiens | 2 | ALA4334435 | subcellular format | Scientific Literature | |
8. | ALA4334835 | Binding | Inhibition of 26S immunoproteasome beta 5 chymotrypsin-like activity in human THP-1 intact cells using Suc-LLVY-AMC as substrate after 2 hrs by luciferase based assay | Homo sapiens | 2 | ALA4334435 | cell-based format | Scientific Literature | |
9. | ALA4334836 | Binding | Inhibition of 26S immunoproteasome beta 5 chymotrypsin-like activity in human HeLa intact cells using Suc-LLVY-AMC as substrate after 2 hrs by luciferase based assay | Homo sapiens | 2 | ALA4334435 | cell-based format | Scientific Literature | |
10. | ALA4334837 | Functional | Inhibition of LPS-induced IL-6 secretion in human PBMC at 10 uM preincubated for 1 hr followed by LPS addition and measured after 24 hrs by cytometric bead array-based FACS analysis | Homo sapiens | 2 | ALA4334435 | cell-based format | Scientific Literature | |
11. | ALA4334838 | Functional | Inhibition of LPS-induced IL-10 secretion in human PBMC at 10 uM preincubated for 1 hr followed by LPS addition and measured after 24 hrs by cytometric bead array-based FACS analysis | Homo sapiens | 2 | ALA4334435 | cell-based format | Scientific Literature | |
12. | ALA4334839 | Functional | Inhibition of LPS-induced IL-1beta production in human PBMC at 10 uM preincubated for 1 hr followed by LPS addition and measured after 24 hrs by cytometric bead array-based FACS analysis | Homo sapiens | 2 | ALA4334435 | cell-based format | Scientific Literature | |
13. | ALA4334840 | Functional | Inhibition of LPS-induced IL-8 production in human PBMC at 10 uM preincubated for 1 hr followed by LPS addition and measured after 24 hrs by cytometric bead array-based FACS analysis | Homo sapiens | 2 | ALA4334435 | cell-based format | Scientific Literature | |
14. | ALA4334841 | Functional | Inhibition of LPS-induced IL-12p70 production in human PBMC at 10 uM preincubated for 1 hr followed by LPS addition and measured after 24 hrs by cytometric bead array-based FACS analysis | Homo sapiens | 2 | ALA4334435 | cell-based format | Scientific Literature | |
15. | ALA4334842 | Functional | Inhibition of LPS-induced TNFalpha production in human PBMC at 10 uM preincubated for 1 hr followed by LPS addition and measured after 24 hrs by cytometric bead array-based FACS analysis | Homo sapiens | 2 | ALA4334435 | cell-based format | Scientific Literature | |
16. | ALA4334844 | Binding | Time-dependent inhibition of human immunoproteasome beta 5i subunit assessed as increase in IC50 value using Suc-LLVY-AMC as substrate co-incubated with substrate without preincubation by fluorescence based analysis relative to 30 mins preincubated compound-treated control | Homo sapiens | 3 | ALA4334435 | single protein format | Scientific Literature | |
17. | ALA4334848 | Binding | Inhibition of 20S immunoproteasome beta 5 chymotrypsin-like activity in human HeLa cell lysates using Suc-LLVY-AMC as substrate preincubated for 30 mins followed by substrate addition and measured for 90 mins by fluorescence based assay | Homo sapiens | 2 | ALA4334435 | subcellular format | Scientific Literature | |
18. | ALA4334851 | Binding | Inhibition of human immunoproteasome beta 2i subunit using Boc-LRR-AMC as substrate preincubated for 30 mins followed by substrate addition measured for 120 mins by fluorescence based assay | Homo sapiens | 10 | ALA4334435 | single protein format | Scientific Literature | |
19. | ALA4334852 | Binding | Inhibition of human immunoproteasome beta 1i subunit using Ac-PAL-AMC as substrate preincubated for 30 mins followed by substrate addition measured for 120 mins by fluorescence based assay | Homo sapiens | 10 | ALA4334435 | single protein format | Scientific Literature | |
20. | ALA4334853 | ADME | Inhibition of human constitutive proteasome beta 5 subunit using Suc-LLVY-AMC as substrate preincubated for 30 mins followed by substrate addition measured for 120 mins by fluorescence based assay | Homo sapiens | 10 | ALA4334435 | single protein format | Scientific Literature |