#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4480297	Binding	Inhibition of DOT1L (2 to 416 residues) (unknown origin) using biotinylated nucleosomes as substrate preincubated for 30 mins followed by substrate addition in presence of [3H-Me]SAM by scintillation proximity assay	Homo sapiens	13	ALA4477274	single protein format	Scientific Literature
2.	ALA4480298	Binding	Binding affinity to human Avi-tagged DOT1L (2 to 416 residues) assessed as target residence time by surface plasmon resonance assay	Homo sapiens	2	ALA4477274	single protein format	Scientific Literature
3.	ALA4480299	Binding	Inhibition of DOT1L in human HeLa cells assessed as reduction in H3K79me2 level after 72 hrs by ELISA	Homo sapiens	2	ALA4477274	cell-based format	Scientific Literature
4.	ALA4480300	Binding	Inhibition of DOT1L in human MOLM13 cells assessed as suppression of HoxA9 gene after 72 hrs by luciferase reporter gene assay	Homo sapiens	2	ALA4477274	cell-based format	Scientific Literature
5.	ALA4480301	Functional	Antiproliferative activity against human MV4-11 cells harboring MLL-AF4 treated for 6 hrs measured after 8 days by Celltiter-Glo reagent based assay	Homo sapiens	2	ALA4477274	cell-based format	Scientific Literature
6.	ALA4480302	ADME	Permeability of compound by HDM-PAMPA assay		2	ALA4477274	assay format	Scientific Literature
7.	ALA4480304	ADME	Clearance in Sprague-Dawley rat at 1 mg/kg, iv administered as bolus dose measured up to 48 hrs by LC-MS/MS analysis	Rattus norvegicus	1	ALA4477274	organism-based format	Scientific Literature
8.	ALA4480305	ADME	Volume of distribution in Sprague-Dawley rat at 1 mg/kg, iv administered as bolus dose measured up to 48 hrs by LC-MS/MS analysis	Rattus norvegicus	1	ALA4477274	organism-based format	Scientific Literature
9.	ALA4480306	ADME	Half life in Sprague-Dawley rat at 1 mg/kg, iv administered as bolus dose by LC-MS/MS analysis	Rattus norvegicus	1	ALA4477274	organism-based format	Scientific Literature
10.	ALA4480307	ADME	AUC (0 to infinity) in Sprague-Dawley rat at 1 mg/kg, iv administered as bolus dose by LC-MS/MS analysis	Rattus norvegicus	1	ALA4477274	organism-based format	Scientific Literature
11.	ALA4480308	ADME	AUC (0 to infinity) in Sprague-Dawley rat at 15 mg/kg, po administered via gastric intubation by LC-MS/MS analysis	Rattus norvegicus	1	ALA4477274	organism-based format	Scientific Literature
12.	ALA4480309	ADME	Cmax in Sprague-Dawley rat at 15 mg/kg, po administered via gastric intubation measured up to 48 hrs by LC-MS/MS analysis	Rattus norvegicus	1	ALA4477274	organism-based format	Scientific Literature
13.	ALA4480310	ADME	Oral bioavailability in Sprague-Dawley rat at 15 mg/kg administered via gastric intubation measured up to 48 hrs by LC-MS/MS analysis	Rattus norvegicus	1	ALA4477274	organism-based format	Scientific Literature
14.	ALA4480311	ADME	Drug level in Sprague-Dawley rat blood at 15 mg/kg, po administered via gastric intubation up to 24 hrs by LC-MS/MS analysis	Rattus norvegicus	1	ALA4477274	organism-based format	Scientific Literature
15.	ALA4480312	ADME	Tmax in Sprague-Dawley rat at 15 mg/kg, po administered via gastric intubation by LC-MS/MS analysis	Rattus norvegicus	1	ALA4477274	organism-based format	Scientific Literature
16.	ALA5138233	Binding	Inhibition of DOT1L (unknown origin)	Homo sapiens	15	ALA5136992	single protein format	Scientific Literature
17.	ALA5138236	Functional	Inhibition of methylation of H3K79 in human MCF-10A cells by fluorescence assay	Homo sapiens	3	ALA5136992	cell-based format	Scientific Literature