| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4346466 | Binding | Binding affinity to GST-tagged recombinant EPAC1 CNBD (169 to 318 residues) (unknown origin) expressed in Escherichia coli BL21 Star (DE3) assessed as relative fluorescence intensity incubated for 4 hrs by 8-NBD-cAMP competition binding assay relative to control | Homo sapiens | 57 | ALA4342525 | assay format | Scientific Literature | |
| 2. | ALA4346467 | Binding | Binding affinity to GST-tagged recombinant EPAC1 CNBD (169 to 318 residues) (unknown origin) expressed in Escherichia coli BL21 Star (DE3) incubated for 4 hrs by 8-NBD-cAMP competition binding assay | Homo sapiens | 6 | ALA4342525 | assay format | Scientific Literature | |
| 3. | ALA4346468 | Binding | Binding affinity to GST-tagged recombinant EPAC1 delta-DEP mutant (149 to 881 residues) (unknown origin) expressed in Escherichia coli BL21 Star (DE3) incubated for 4 hrs by 8-NBD-cAMP competition binding assay | Homo sapiens | 4 | ALA4342525 | assay format | Scientific Literature | |
| 4. | ALA4346476 | Binding | Activation of EPAC1 (unknown origin) expressed in U2OS cells at 100 uM incubated for 10 mins using GST-RalGDS-RBD by Western blot analysis based active GTP-bound Rap1 pull down assay | Homo sapiens | 6 | ALA4342525 | cell-based format | Scientific Literature | |
| 5. | ALA4346477 | Binding | Activation of EPAC1 in HUVEC assessed as reduction in IL-6-promoted signaling by measuring pSTAT3 protein levels at 100 uM pre-incubated for 30 mins before IL6 and sIL6Ralpha stimulation for 5 hrs by Western blotting analysis | Homo sapiens | 5 | ALA4342525 | cell-based format | Scientific Literature | |
| 6. | ALA4346478 | Binding | Activation of EPAC1 in HUVEC assessed as reduction in IL-6-promoted signaling by measuring pERK protein levels at 100 uM pre-incubated for 30 mins before IL6 and sIL6Ralpha stimulation for 5 hrs by Western blotting analysis | Homo sapiens | 5 | ALA4342525 | cell-based format | Scientific Literature | |
| 7. | ALA4346480 | Binding | Activation of EPAC1 in HUVEC assessed as reduction in IL-6-promoted signaling by measuring VCAM1 protein expression at 100 uM pre-incubated for 30 mins before IL6 and sIL6Ralpha stimulation for 5 hrs by Western blotting analysis | Homo sapiens | 5 | ALA4342525 | cell-based format | Scientific Literature | |
| 8. | ALA4346483 | Binding | Activity at EPAC1 in HUVEC assessed as promotion of IL-6-promoted signaling by measuring increased pAKT protein levels at 100 uM pre-incubated for 30 mins before IL6 and sIL6Ralpha stimulation for 5 hrs by Western blotting analysis | Homo sapiens | 2 | ALA4342525 | cell-based format | Scientific Literature | |
| 9. | ALA4605939 | Binding | Binding affinity to GST-tagged human EPAC1 CNBD (149 to 318 residues) expressed in Escherichia coli BL21 (DE3) incubated for 30 mins by 8-NBD-cAMP based competitive fluorescence assay | Homo sapiens | 2 | ALA4602718 | assay format | Scientific Literature | |
| 10. | ALA4605940 | Binding | Binding affinity to GST-tagged human EPAC1 L273W mutant expressed in Escherichia coli BL21 (DE3) incubated for 30 mins by 8-NBD-cAMP based competitive fluorescence assay | Homo sapiens | 1 | ALA4602718 | assay format | Scientific Literature | |
| 11. | ALA4605941 | Binding | Binding affinity to GST-tagged human EPAC1 CNBD (149 to 318 residues) expressed in Escherichia coli BL21 (DE3) incubated for 30 mins by 1H,15N-HSQC NMR spectroscopy | Homo sapiens | 1 | ALA4602718 | assay format | Scientific Literature | |
| 12. | ALA4605942 | Binding | Binding affinity to GST-tagged human EPAC1 CNBD (149 to 318 residues)expressed in Escherichia coli BL21 (DE3) assessed as compound binding to cAMP-binding sites incubated for 30 mins by CHESPA method | Homo sapiens | 1 | ALA4602718 | assay format | Scientific Literature | |
| 13. | ALA4605943 | Binding | Binding affinity to GST-tagged human EPAC1 CNBD (149 to 318 residues)expressed in Escherichia coli BL21 (DE3) assessed as compound binding to phosphate binding cassette region incubated for 30 mins by CHESPA method | Homo sapiens | 1 | ALA4602718 | assay format | Scientific Literature | |
| 14. | ALA4605944 | Binding | Binding affinity to GST-tagged human EPAC1 CNBD (149 to 318 residues)expressed in Escherichia coli BL21 (DE3) assessed as compound binding to base binding region incubated for 30 mins by CHESPA method | Homo sapiens | 1 | ALA4602718 | assay format | Scientific Literature | |
| 15. | ALA4605945 | Binding | Binding affinity to GST-tagged human EPAC1 CNBD (149 to 318 residues)expressed in Escherichia coli BL21 (DE3) assessed as induction of conformational changes in compound by NOESY NMR spectroscopy | Homo sapiens | 1 | ALA4602718 | assay format | Scientific Literature | |
| 16. | ALA4605946 | Binding | Binding affinity to GST-tagged human EPAC1 CNBD (149 to 318 residues)expressed in Escherichia coli BL21 (DE3) assessed as induction Intermolecular NOEs peaks in PBC and BBR residues by 15N-NOESY-HSQC NMR spectroscopy | Homo sapiens | 4 | ALA4602718 | assay format | Scientific Literature | |
| 17. | ALA4605947 | Binding | Binding affinity to GST-tagged human EPAC1 CNBD (149 to 318 residues)expressed in Escherichia coli BL21 (DE3) by STD and guanidinium HSQC NMR analysis | Homo sapiens | 1 | ALA4602718 | assay format | Scientific Literature | |
| 18. | ALA4605948 | Binding | Binding affinity to GST-tagged human EPAC1 CNBD (149 to 318 residues)expressed in Escherichia coli BL21 (DE3) assessed as binding to R279 Guanidinium residue by guanidinium HSQC NMR spectroscopy | Homo sapiens | 2 | ALA4602718 | assay format | Scientific Literature | |
| 19. | ALA4605949 | Binding | Binding affinity to GST-tagged human EPAC1 CNBD (149 to 318 residues)expressed in Escherichia coli BL21 (DE3) assessed as induction of perturbation of allosteric network sites by CHESCA method | Homo sapiens | 1 | ALA4602718 | assay format | Scientific Literature |
The page will load shortly, Thanks for your patience!