#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4421085	B	Inhibition of N-terminal 6x-His-tagged recombinant human SOS1 catalytic domain (residues 564 to 1049 residues) expressed in Escherichia coli BL21 (DE3) assessed as reduction in 50 nM SOS1-mediated GDP/GTP nucleotide exchange upon 2 uM N-terminal-GST-tagged H-Ras (1 to 166 residues) by BODIPY-FL-GDP dissociation assay	Homo sapiens	36		assay format	Patent Bioactivity Data
2.	ALA4421087	B	Binding affinity to N-terminal 6x-His-tagged recombinant human SOS1 catalytic domain (residues 564 to 1049 residues) expressed in Escherichia coli BL21 (DE3) by label-free microscale thermophoresis analysis	Homo sapiens	4		assay format	Patent Bioactivity Data
3.	ALA4421088	B	Binding affinity to N-terminal 6x-His-tagged recombinant human SOS1 catalytic domain (residues 564 to 1049 residues) expressed in Escherichia coli BL21 (DE3) assessed as reduction in N-terminal-GST-tagged H-Ras (1 to 166 residues) binding to NT-647 cysteine-labeled purified SOS1 catalytic domain by competitive microscale thermophoresis analysis	Homo sapiens	4		assay format	Patent Bioactivity Data
4.	ALA4421101	B	Inhibition of SOS1 in human DU145 cells assessed as reduction in cell proliferation at 20 to 60 uM incubated for 3 days and measured every 24 hrs by MTS assay	Homo sapiens	2		cell-based format	Patent Bioactivity Data
5.	ALA4421123	B	Inhibition of N-terminal 6x-His-tagged recombinant human SOS1 catalytic domain (residues 564 to 1049 residues) expressed in Escherichia coli BL21 (DE3) assessed as reduction in 50 nM SOS1-mediated GTP loading of 2 uM N-terminal-GST-tagged H-Ras (1 to 166 residues) by BODIPY-TR-GTP GTP loading exchange assay	Homo sapiens	2		assay format	Patent Bioactivity Data
6.	ALA4421124	B	Binding affinity to NT-647 cysteine-labeled and N-terminal-GST-tagged recombinant human H-Ras (1 to 166 residues) expressed in Escherichia coli BL21 (DE3) at up to 100 uM by microscale thermophoresis analysis	Homo sapiens	3		assay format	Patent Bioactivity Data
7.	ALA4421125	B	Inhibition of SOS1 in overnight serum-starved mouse NIH/3T3 cells assessed as effect on Ras-GTP levels at 20 to 40 uM pre-incubated for 16 hrs before EGF stimulation for 5 mins by GST-Raf1 effector domain pulldown assay	Mus musculus	1		assay format	Patent Bioactivity Data
8.	ALA4421126	B	Inhibition of SOS1 in overnight serum-starved mouse NIH/3T3 cells assessed as reduction in ERK1/2 phosphorylation at 20 to 40 uM pre-incubated for 16 hrs before EGF stimulation for 5 mins by Western blot analysis	Mus musculus	1		assay format	Patent Bioactivity Data
9.	ALA4421127	B	Inhibition of SOS1 in overnight serum-starved mouse NIH/3T3 cells assessed as reduction in Akt phosphorylation at 20 to 40 uM pre-incubated for 16 hrs before EGF stimulation for 5 mins by Western blot analysis	Mus musculus	1		assay format	Patent Bioactivity Data
10.	ALA4421132	B	Inhibition of SOS1 in overnight serum-starved human DU145 cells assessed as reduction in ERK1 phosphorylation at 20 to 40 uM pre-incubated for 16 hrs before EGF stimulation for 5 mins by Western blot analysis	Homo sapiens	2		assay format	Patent Bioactivity Data
11.	ALA4421133	B	Inhibition of N-terminal 6x-His-tagged recombinant human SOS1 catalytic domain (residues 564 to 1049 residues) expressed in Escherichia coli BL21 (DE3) assessed as reduction in 50 nM SOS1-mediated GDP/GTP nucleotide exchange upon 2 uM N-terminal-GST-tagged H-Ras (1 to 166 residues) at 10 uM by BODIPY-FL-GDP dissociation assay	Homo sapiens	1		assay format	Patent Bioactivity Data
12.	ALA4421136	B	Inhibition of N-terminal 6x-His-tagged recombinant human ITSN1 DH and PH domain (1227 to 1571 residues) expressed in Escherichia coli BL21 (DE3) assessed as reduction in 50 nM SOS1-mediated GDP/GTP nucleotide exchange upon 2 uM N-terminal-GST-tagged Cdc42 (1 to 182 residues) at 10 uM by BODIPY-FL-GDP dissociation assay	Homo sapiens	1		assay format	Patent Bioactivity Data
13.	ALA4421138	B	Inhibition of SOS1 in overnight serum-starved mouse NIH/3T3 cells assessed as reduction in cell proliferation at 20 to 60 uM incubated for 3 days and measured every 24 hrs by MTS assay	Mus musculus	1		assay format	Patent Bioactivity Data