# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4376141 | Binding | Inhibition of recombinant human SMYD2 (1 to 433 residues) expressed in baculovirus infected Sf9 insect cells using biotinylated-p53 peptide (361 to 380 residues) as substrate measured after 50 mins in presence of 70 nM [3H]-SAM by scintillation proximity assay | Homo sapiens | 7 | ALA4373722 | cell-based format | Scientific Literature | |
2. | ALA4376143 | Binding | Inhibition of recombinant human SMYD2 (1 to 433 residues) expressed in baculovirus infected Sf9 insect cells using biotinylated-p53 peptide (361 to 380 residues) as substrate measured after 120 mins in presence of 20 uM [3H]-SAM by scintillation proximity assay | Homo sapiens | 6 | ALA4373722 | cell-based format | Scientific Literature | |
3. | ALA4376144 | Binding | Inhibition of V5-tagged SMYD2 (unknown origin) transfected in human MCF7 cells co-transfected with FLAG-tagged p53 assessed as reduction in p53 methylation at K370 residue at 30 uM measured after 20 hrs by immunoblot analysis | Homo sapiens | 1 | ALA4373722 | cell-based format | Scientific Literature | |
4. | ALA4376145 | ADME | Effect on total p53 level in human MCF7 cells co-transfected with V5-tagged SMYD2/FLAG-tagged p53 up to 30 uM measured after 20 hrs by immunoblot analysis | Homo sapiens | 1 | ALA4373722 | cell-based format | Scientific Literature |