# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4408439 | Functional | Antiproliferative activity against human NCI-H1703 cells expressing FASN assessed as growth inhibition at 25 uM after 72 hrs by MTS assay relative to control | Homo sapiens | 13 | ALA4406830 | cell-based format | Scientific Literature | |
2. | ALA4408450 | Functional | Induction of apoptosis in human NCI-H1703 cells expressing FASN assessed as cell death at 5 to 100 uM after 72 hrs by Annexin-V FITC staining/PI-based flow cytometry | Homo sapiens | 1 | ALA4406830 | cell-based format | Scientific Literature | |
3. | ALA4408451 | Functional | Induction of apoptosis in human PC9 cells harboring FASN expression and EGFR delE746-A750 mutant assessed as cell death at 5 to 100 uM after 72 hrs by Annexin-V FITC/PI staining-based flow cytometry | Homo sapiens | 1 | ALA4406830 | cell-based format | Scientific Literature | |
4. | ALA4408455 | Binding | Inhibition of full length human FASN transfected in HeLa cell lysates at 10 uM using acetyl Co-A/malonyl Co-A as substrate preincubated with enzyme for 2 hrs followed by substrate addition measured after 10 mins in presence of NADPH by microplate reader analysis | Homo sapiens | 4 | ALA4406830 | subcellular format | Scientific Literature | |
5. | ALA4408461 | Binding | Inhibition of FASN in human PC9 cells harboring FASN expression and EGFR delE746-A750 mutant assessed as disruption of AKT/FASN positive feedback loop by measuring decrease in FASN expression at 5 uM after 48 hrs by Western immunoblot based densitometric assay relative to control | Homo sapiens | 3 | ALA4406830 | cell-based format | Scientific Literature | |
6. | ALA4408462 | Binding | Inhibition of FASN in human PC9 cells harboring FASN expression and EGFR delE746-A750 mutant assessed as disruption of AKT/FASN positive feedback loop by measuring decrease in FASN expression at 50 uM after 48 hrs by Western immunoblot based densitometric assay relative to control | Homo sapiens | 1 | ALA4406830 | cell-based format | Scientific Literature | |
7. | ALA4408463 | Binding | Inhibition of FASN in human PC9 cells harboring FASN expression and EGFR delE746-A750 mutant assessed as disruption of AKT/FASN positive feedback loop by measuring decrease in FASN expression at 100 uM after 48 hrs by Western immunoblot based densitometric assay relative to control | Homo sapiens | 2 | ALA4406830 | cell-based format | Scientific Literature | |
8. | ALA4408465 | Binding | Inhibition of FASN in human NCI-H1703 cells expressing FASN assessed as disruption of AKT/FASN positive feedback loop by measuring decrease in FASN expression at 5 uM after 48 hrs by Western immunoblot based densitometric assay relative to control | Homo sapiens | 3 | ALA4406830 | cell-based format | Scientific Literature | |
9. | ALA4408466 | Binding | Inhibition of FASN in human NCI-H1703 cells expressing FASN assessed as disruption of AKT/FASN positive feedback loop by measuring decrease in FASN expression at 50 uM after 48 hrs by Western immunoblot based densitometric assay relative to control | Homo sapiens | 1 | ALA4406830 | cell-based format | Scientific Literature | |
10. | ALA4408467 | Binding | Inhibition of FASN in human NCI-H1703 cells expressing FASN assessed as disruption of AKT/FASN positive feedback loop by measuring decrease in FASN expression at 100 uM after 48 hrs by Western immunoblot based densitometric assay relative to control | Homo sapiens | 1 | ALA4406830 | cell-based format | Scientific Literature | |
11. | ALA4408469 | Binding | Inhibition of FASN in human PC9 cells harboring FASN expression and EGFR delE746-A750 mutant assessed as disruption of AKT/FASN positive feedback loop by measuring reduction in AKT expression at 5 uM after 48 hrs by Western immunoblot based densitometric assay relative to control | Homo sapiens | 3 | ALA4406830 | cell-based format | Scientific Literature | |
12. | ALA4408470 | Binding | Inhibition of FASN in human PC9 cells harboring FASN expression and EGFR delE746-A750 mutant assessed as disruption of AKT/FASN positive feedback loop by measuring reduction in AKT expression at 50 uM after 48 hrs by Western immunoblot based densitometric assay relative to control | Homo sapiens | 1 | ALA4406830 | cell-based format | Scientific Literature | |
13. | ALA4408471 | Binding | Inhibition of FASN in human PC9 cells harboring FASN expression and EGFR delE746-A750 mutant assessed as disruption of AKT/FASN positive feedback loop by measuring reduction in AKT expression at 100 uM after 48 hrs by Western immunoblot based densitometric assay relative to control | Homo sapiens | 1 | ALA4406830 | cell-based format | Scientific Literature | |
14. | ALA4408473 | Binding | Inhibition of FASN in human NCI-H1703 cells expressing FASN assessed as disruption of AKT/FASN positive feedback loop by measuring decrease in AKT expression at 5 uM after 48 hrs by Western immunoblot based densitometric assay relative to control | Homo sapiens | 3 | ALA4406830 | cell-based format | Scientific Literature | |
15. | ALA4408474 | Binding | Inhibition of FASN in human NCI-H1703 cells expressing FASN assessed as disruption of AKT/FASN positive feedback loop by measuring decrease in AKT expression at 50 uM after 48 hrs by Western immunoblot based densitometric assay relative to control | Homo sapiens | 1 | ALA4406830 | cell-based format | Scientific Literature | |
16. | ALA4408475 | Binding | Inhibition of FASN in human NCI-H1703 cells expressing FASN assessed as disruption of AKT/FASN positive feedback loop by measuring decrease in AKT expression at 100 uM after 48 hrs by Western immunoblot based densitometric assay relative to control | Homo sapiens | 1 | ALA4406830 | cell-based format | Scientific Literature | |
17. | ALA4408477 | Binding | Inhibition of FASN in human PC9 cells harboring FASN expression and EGFR delE746-A750 mutant assessed as disruption of AKT/FASN positive feedback loop by measuring reduction in phosphorylated AKT expression at 5 uM after 48 hrs by Western immunoblot based densitometric assay relative to control | Homo sapiens | 3 | ALA4406830 | cell-based format | Scientific Literature | |
18. | ALA4408478 | Binding | Inhibition of FASN in human PC9 cells harboring FASN expression and EGFR delE746-A750 mutant assessed as disruption of AKT/FASN positive feedback loop by measuring reduction in phosphorylated AKT expression at 50 uM after 48 hrs by Western immunoblot based densitometric assay relative to control | Homo sapiens | 1 | ALA4406830 | cell-based format | Scientific Literature | |
19. | ALA4408479 | Binding | Inhibition of FASN in human PC9 cells harboring FASN expression and EGFR delE746-A750 mutant assessed as disruption of AKT/FASN positive feedback loop by measuring reduction in phosphorylated AKT expression at 100 uM after 48 hrs by Western immunoblot based densitometric assay relative to control | Homo sapiens | 1 | ALA4406830 | cell-based format | Scientific Literature | |
20. | ALA4408481 | Binding | Inhibition of FASN in human NCI-H1703 cells expressing FASN assessed as disruption of AKT/FASN positive feedback loop by measuring decrease in phosphorylated AKT expression at 5 uM after 48 hrs by Western immunoblot based densitometric assay relative to control | Homo sapiens | 3 | ALA4406830 | cell-based format | Scientific Literature |