| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4345047 | B | Displacement of 2FAMInsP5 from recombinant human N-terminal His-tagged SHIP2 (419 to 832 residues) expressed in Escherichia coli Rosetta2 (DE3) cells by fluorescence polarization assay | Homo sapiens | 6 | cell-based format | Scientific Literature | ||
| 2. | ALA4345048 | B | Binding affinity to recombinant human N-terminal His-tagged SHIP2 (419 to 832 residues) expressed in Escherichia coli Rosetta2 (DE3) cells assessed as increase in release of phosphate at 100 uM incubated for 10 mins by phosphate release assay | Homo sapiens | 6 | cell-based format | Scientific Literature | ||
| 3. | ALA4345049 | B | Displacement of Ins(1,3,4,5)P4 from recombinant human N-terminal His-tagged SHIP2 (419 to 832 residues) expressed in Escherichia coli BL21 (DE3) | Homo sapiens | 2 | assay format | Scientific Literature | ||
| 4. | ALA4345050 | B | Inhibition of His-tagged SHIP2 (unknown origin) incubated for 5 mins using Ins(1,3,4,5)P4 as substrate by malachite green reagent based phosphate assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 5. | ALA4345051 | B | Inhibition of recombinant human C-terminal His-tagged INPP5B (259 to 563 residues) expressed in Escherichia coli BL21 (DE3) incubated for 5 mins using Ins(1,4,5)P3 as substrate by malachite green reagent based phosphate assay | Homo sapiens | 2 | assay format | Scientific Literature | ||
| 6. | ALA4345052 | B | Inhibition of recombinant human brain INPP5A expressed in Escherichia coli incubated for 10 mins by malachite green reagent based phosphate assay | Homo sapiens | 6 | assay format | Scientific Literature | ||
| 7. | ALA4345053 | B | Substrate activity at recombinant human N-terminal His-tagged SHIP2 (419 to 832 residues) expressed in Escherichia coli Rosetta2 (DE3) cells assessed as 5-OH-Bz(1,2,4)P3 product formation at 100 uM incubated for 12 hrs by fluorescence based HPLC analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 8. | ALA4345054 | B | Substrate activity at recombinant human N-terminal His-tagged SHIP2 (419 to 832 residues) expressed in Escherichia coli Rosetta2 (DE3) cells assessed as 5-OH-Bz(1,2,4)P3 product formation at 100 uM incubated for 2 hrs with 100 nM to 1 mM of SHIP2 by fluorescence based HPLC analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 9. | ALA4345055 | B | Substrate activity at recombinant human N-terminal His-tagged SHIP2 (419 to 832 residues) expressed in Escherichia coli Rosetta2 (DE3) cells assessed as 5-OH-Bz(1,2,4)P3 product formation at 100 uM measured upto 4 hrs by fluorescence based HPLC analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 10. | ALA4345056 | B | Substrate activity at recombinant human N-terminal His-tagged SHIP2 (419 to 832 residues) expressed in Escherichia coli Rosetta2 (DE3) cells assessed as 5-OH-Bz(1,2,4)P3 product formation at 100 uM incubated for 2 hrs by fluorescence based HPLC analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 11. | ALA4345057 | B | Substrate activity at recombinant human N-terminal His-tagged SHIP2 (419 to 832 residues) expressed in Escherichia coli Rosetta2 (DE3) cells assessed as 5-OH-Bz(1,2,4)P3 product formation at 100 uM incubated for 2 hrs in presence of INS(1,3,4,5)P4 by fluorescence based HPLC analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 12. | ALA4345058 | B | Substrate activity at recombinant human N-terminal His-tagged SHIP2 (419 to 832 residues) expressed in Escherichia coli Rosetta2 (DE3) cells assessed as 5-OH-Bz(1,2,4)P3 product formation at 100 uM incubated for 2 hrs in presence of AS1949490 by fluorescence based HPLC analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 13. | ALA5053568 | B | Inhibition of SHIP2 in serum-starved mouse Mm1 cells assessed as reduction in Akt phosphorylation incubated for 30 mins by Western blot analysis | Mus musculus | 3 | tissue-based format | Scientific Literature |
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