# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4380517 | B | Inhibition of recombinant full length human N-terminal His-tagged TBK1 using biotin-labelled Ahx-GDEDFSSFAEPG peptide as substrate preincubated with enzyme for 15 mins followed by substrate addition and further incubated for 30 mins in presence of 10 uM of ATP by TR-FRET assay | Homo sapiens | 37 | single protein format | Scientific Literature | ||
2. | ALA4380518 | B | Inhibition of recombinant full length human N-terminal His-tagged TBK1 using biotin-labelled Ahx-GDEDFSSFAEPG peptide as substrate preincubated with enzyme for 15 mins followed by substrate addition and further incubated for 30 mins in presence of 1 mM of ATP by TR-FRET assay | Homo sapiens | 37 | single protein format | Scientific Literature | ||
3. | ALA4380519 | B | Inhibition of recombinant full length human C-terminal GST-tagged IKKepsilon expressed in baculovirus expression system using biotin-labelled Ahx-GDEDFSSFAEPG peptide as substrate preincubated with enzyme for 15 mins followed by substrate addition and further incubated for 30 mins in presence of 10 uM of ATP by TR-FRET assay | Homo sapiens | 37 | assay format | Scientific Literature | ||
4. | ALA4380520 | B | Inhibition of IKKepsilon/TBK1 in human MDB-MB-231 cells transduced with lentiviral vector expressing human IRF3 assessed as decrease in poly(I:C)-stimulated IRF3 phosphorylation at S385/386 residues preincubated for 1 hr followed by poly(I:C) addition and measured after 1 hr | Homo sapiens | 37 | cell-based format | Scientific Literature | ||
5. | ALA4380521 | F | Antiproliferative activity against human SK-MEL-2 cells harboring NRAS/TP53 mutation assessed as reduction in cell viability after 96 hrs by CellTiter-Glo luminescence assay | Homo sapiens | 37 | cell-based format | Scientific Literature | ||
6. | ALA4380522 | F | Antiproliferative activity against human ACHN cells harboring CDKN2A mutation assessed as reduction in cell viability after 96 hrs by CellTiter-Glo luminescence assay | Homo sapiens | 37 | cell-based format | Scientific Literature | ||
7. | ALA4380523 | B | Inhibition of recombinant full length human His-tagged CDK9/CyclinT1 expressed in baculovirus expression system using biotin-labelled Ttds-YISPLKSPYKISEG peptide as substrate preincubated with enzyme for 15 mins followed by substrate addition and further incubated for 25 mins presence of 10 uM of ATP by TR-FRET assay | Homo sapiens | 22 | assay format | Scientific Literature | ||
8. | ALA4380524 | B | Inhibition of recombinant human N-terminal GST-tagged FLT3 (564 to end residues) expressed in sf21 cells using biotin labelled Ahx-GGEEEEYFELVKKKK peptide as substrate preincubated with enzyme for 15 mins followed by substrate addition and further incubated for 45 mins in presence of 10 uM of ATP by TR-FRET assay | Homo sapiens | 20 | cell-based format | Scientific Literature | ||
9. | ALA4380525 | B | Inhibition of recombinant full length human His6-tagged RSK4 expressed in baculovirus infected sf21 cells using biotin labelled Ahx-KKLNRTLSFAEPG peptide as substrate preincubated with enzyme for 15 mins followed by substrate addition and further incubated for 30 mins in presence of 10 uM of ATP by TR-FRET assay | Homo sapiens | 20 | cell-based format | Scientific Literature | ||
10. | ALA4380526 | B | Inhibition of wild-type human partial length DRAK1 (R32 to E363 residues) expressed in bacterial expression system by Kinomescan method | Homo sapiens | 20 | assay format | Scientific Literature | ||
11. | ALA4380527 | B | Inhibition of wild-type human partial length ULK1 (M1 to T309 residues) expressed in mammalian expression system by Kinomescan method | Homo sapiens | 20 | assay format | Scientific Literature | ||
12. | ALA4380561 | B | Selectivity index, ratio of IC50 for wild-type human partial length ULK1 (M1 to T309 residues) expressed in mammalian expression system by Kinomescan method to IC50 for inhibition of recombinant full length human N-terminal His-tagged TBK1 using biotin-labelled Ahx-GDEDFSSFAEPG peptide as substrate preincubated with enzyme for 15 mins followed by substrate addition and further incubated for 30 mins in presence of 1 mM of ATP by TR-FRET assay | Homo sapiens | 1 | assay format | Scientific Literature |