# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4415176 | B | Antagonist activity at SMO in mouse NIH/3T3 cells harboring Gli-responsive firefly luciferase gene assessed as inhibition of SAG-induced response preincubated for 2 hrs followed by SAG addition and measured after 24 hrs by Bright-Glo luciferase assay | Mus musculus | 14 | ALA4414554 | cell-based format | Scientific Literature | |
2. | ALA4415177 | B | Displacement of BODIPY-LY from SMO (unknown origin) expressed in HEK293F cells measured after 15 mins by flow cytometric analysis | Homo sapiens | 6 | ALA4414554 | cell-based format | Scientific Literature | |
3. | ALA4415180 | B | Binding affinity to recombinant SMO Gly527 residue (unknown origin) assessed as modification of INLFAMFGTGIAMSTWVWTKATLLIWRR peptide at 20 uM preincubated for 3 hrs followed by UV irradiation for 15 mins by MS/MS analysis | Homo sapiens | 1 | ALA4414554 | single protein format | Scientific Literature | |
4. | ALA4415181 | B | Binding affinity to recombinant SMO Thr528 residue (unknown origin) assessed as modification of INLFAMFGTGIAMSTWVWTKATLLIWRR peptide at 20 uM preincubated for 3 hrs followed by UV irradiation for 15 mins by MS/MS analysis | Homo sapiens | 1 | ALA4414554 | single protein format | Scientific Literature | |
5. | ALA4415182 | B | Binding affinity to recombinant SMO Gly529 residue (unknown origin) assessed as modification of INLFAMFGTGIAMSTWVWTKATLLIWRR peptide at 20 uM preincubated for 3 hrs followed by UV irradiation for 15 mins by MS/MS analysis | Homo sapiens | 1 | ALA4414554 | single protein format | Scientific Literature | |
6. | ALA4415183 | B | Binding affinity to recombinant SMO Ala345 residue (unknown origin) at 20 uM preincubated for 3 hrs followed by UV irradiation for 15 mins by MS/MS analysis | Homo sapiens | 1 | ALA4414554 | single protein format | Scientific Literature | |
7. | ALA4415184 | B | Binding affinity to recombinant SMO Thr349 residue (unknown origin) at 20 uM preincubated for 3 hrs followed by UV irradiation for 15 mins by MS/MS analysis | Homo sapiens | 1 | ALA4414554 | single protein format | Scientific Literature | |
8. | ALA4415185 | B | Binding affinity to recombinant SMO Glu158 residue (unknown origin) at 20 uM preincubated for 3 hrs followed by UV irradiation for 15 mins by MS/MS analysis | Homo sapiens | 1 | ALA4414554 | single protein format | Scientific Literature | |
9. | ALA4415186 | B | Binding affinity to recombinant SMO His103 residue (unknown origin) at 20 uM preincubated for 3 hrs followed by UV irradiation for 15 mins by MS/MS analysis | Homo sapiens | 1 | ALA4414554 | single protein format | Scientific Literature |