# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4372727 | B | Inhibition of recombinant His6-tagged Kif15 (N700 residues) (unknown origin) expressed in HeLa cells assessed as reduction in Alexa-594 labelled microtubule gliding measured every 2 secs for 20 secs by epifluorescence microscopic analysis | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
2. | ALA4372728 | B | Reversible inhibition of recombinant His6-tagged Kif15 (N700 residues) (unknown origin) expressed in HeLa cells assessed as reduction in Alexa-594 labelled microtubule gliding at 10 uM treated for 1 min followed by compound washout and measured every 2 secs for 1 min by epifluorescence microscopic analysis | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
3. | ALA4372733 | B | Inhibition of Kif15 in TP53-deficient human RPE1 cells assessed as cells with bipolar pre-anaphase microtubule structure at 25 uM after 24 hrs by Hoechst 33342 staining based immunofluorescence microscopic analysis (Rvb = 90 to 94%) | Homo sapiens | 2 | assay format | Scientific Literature | ||
4. | ALA4372734 | B | Inhibition of Kif15 in TP53-deficient human RPE1 cells assessed as reduction in spindle-bound Kif15 level at 25 uM after 24 hrs by Hoechst 33342 staining based immunofluorescence microscopic analysis relative to control | Homo sapiens | 2 | assay format | Scientific Literature | ||
5. | ALA4372735 | B | Inhibition of Kif15 in TP53-deficient human RPE1 cells assessed as reduction in Kif15 level at 25 uM after 24 hrs by Western blot analysis relative to control | Homo sapiens | 2 | assay format | Scientific Literature | ||
6. | ALA4372736 | B | Inhibition of Kif15 in TP53-deficient human RPE1 cells assessed as effect on spindle length at 25 uM after 24 hrs by Hoechst 33342 staining based immunofluorescence microscopic analysis (Rvb = 11.4 +/- 0.2 um) | Homo sapiens | 2 | assay format | Scientific Literature | ||
7. | ALA4372737 | B | Inhibition of Kif15 in K51-resistant human KIRC1 cells assessed as cells with monopolar spindles at 25 uM after 24 hrs by Hoechst 33342 staining based immunofluorescence microscopic analysis (Rvb = 43 to 49%) | Homo sapiens | 2 | assay format | Scientific Literature | ||
8. | ALA4372740 | B | Inhibition of recombinant C-terminal truncated His6-tagged Kif15 (N700 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) assessed as reduction in microtubule gliding measured up to 2 mins | Homo sapiens | 1 | assay format | Scientific Literature | ||
9. | ALA4372743 | B | ATP competitive inhibition of recombinant His6-tagged Kif15 ATPase activity (N700 residues) (unknown origin) expressed in HeLa cells at 5 uM using microtubule as substrate preincubated for 30 mins followed by varying levels of ATP addition by double reciprocal Lineweaver-Burk plot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature |