# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4349137 | Binding | Irreversible inhibition of 1-(4-((2-((4-(4-(but-3-yn-1-yl)piperazin-1-yl)phenyl)amino)-5-(6-(methylamino)pyrazin-2-yl)-7H-pyrrolo [2,3-d]pyrimidin-4-yl)oxy)piperidin-1-yl)prop-2-en-1-one probe binding to recombinant human ITK at 20 uM preincubated for 2 hrs followed by probe addition and measured after 3 hrs by TAMRA azide-based fluorescence assay | Homo sapiens | 3 | ALA4346710 | single protein format | Scientific Literature | |
2. | ALA4349187 | Binding | Inhibition of 1-(4-((2-((4-(4-(but-3-yn-1-yl)piperazin-1-yl)phenyl)amino)-5-(6-(methylamino)pyrazin-2-yl)-7H-pyrrolo [2,3-d]pyrimidin-4-yl)oxy)piperidin-1-yl)prop-2-en-1-one probe binding to ITK in human Jurkat cells at 10 uM preincubated for 3 hrs followed by probe addition and measured after 3 hrs by immunoblot analysis | Homo sapiens | 2 | ALA4346710 | cell-based format | Scientific Literature | |
3. | ALA4603777 | Binding | Inhibition of ITK (unknown origin) | Homo sapiens | 1 | ALA4602634 | single protein format | Scientific Literature | |
4. | ALA4603781 | Binding | Irreversible inhibition of binding of N-(2-((6-((but-3-yn-1-yloxy) (phenyl)methyl)-2-(6-(difluoromethyl)-1H-indazol-3-yl)-1H-indol-4-yl)oxy)ethyl)acrylamide to recombinant ITK (unknown origin) at 50 uM pretreated with compound prior to probe addition by fluorescence scan analysis | Homo sapiens | 4 | ALA4602634 | single protein format | Scientific Literature | |
5. | ALA4603822 | Binding | Inhibition of ITK in anti-CD3/CD28 beads-stimulated human Jurkat cells assessed as reduction in Plcgamma1 phosphorylation incubated for 2 hrs followed by stimulation with anti-CD3/CD28 beads by Western blot analysis | Homo sapiens | 3 | ALA4602634 | cell-based format | Scientific Literature | |
6. | ALA4603825 | Binding | Irreversible inhibition of ITK in anti-CD3/CD28 beads-stimulated human Jurkat cells assessed as reduction in Plcgamma1 phosphorylation at 3 uM incubated for 2 hrs followed by compound washout with PBS and later stimulated with antiCD3/CD28 for 15 mins by Western blot analysis | Homo sapiens | 4 | ALA4602634 | cell-based format | Scientific Literature |