#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4419454	B	Inhibition of ferroportin in mouse J774 cells assessed as reduction in binding of hepcidin to ferroportin pre-incubated for 15 mins before TMR-hepcidin addition and further incubated for 2 hrs by fluorescence based hepcidin internalization assay	Mus musculus	194		cell-based format	Patent Bioactivity Data
2.	ALA4419455	B	Binding affinity to C-terminal FLAG-tagged human ferroportin expressed in Pichia pastoris assessed as reduction in inhibition of binding of TMR-hepcidin to Fpn incubated for 90 mins by fluorescence polarization assay	Homo sapiens	185		assay format	Patent Bioactivity Data
3.	ALA4419456	B	Inhibition of GFP-tagged human ferroportin expressed in HEK293 #354 cells assessed as reduction in Fpn-mediated iron efflux incubated overnight by iron regulatory element -driven beta-lactamase reporter gene assay	Homo sapiens	130		cell-based format	Patent Bioactivity Data
4.	ALA4419480	F	Induction of hypoferremia in wild type C57BL/6 mouse assessed as decrease in serum iron levels at 10 mg/kg, po measured after 3 hrs post dose	Mus musculus	23		organism-based format	Patent Bioactivity Data
5.	ALA4419481	F	Induction of hypoferremia in wild type C57BL/6 mouse assessed as decrease in serum iron levels at 30 mg/kg, po measured after 3 hrs post dose	Mus musculus	23		organism-based format	Patent Bioactivity Data
6.	ALA4419482	F	Induction of hypoferremia in wild type C57BL/6 mouse assessed as decrease in serum iron levels at 100 mg/kg, po measured after 3 hrs post dose	Mus musculus	23		organism-based format	Patent Bioactivity Data