#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4421084	B	Inhibition of N-terminal 6x-His-tagged recombinant human SOS1 catalytic domain (residues 564 to 1049 residues) expressed in Escherichia coli BL21 (DE3) assessed as reduction in 50 nM SOS1-mediated GDP/GTP nucleotide exchange upon 2 uM N-terminal-GST-tagged H-Ras (1 to 166 residues) at 100 uM by BODIPY-FL-GDP dissociation assay	Homo sapiens	5		assay format	Patent Bioactivity Data
2.	ALA4421088	B	Binding affinity to N-terminal 6x-His-tagged recombinant human SOS1 catalytic domain (residues 564 to 1049 residues) expressed in Escherichia coli BL21 (DE3) assessed as reduction in N-terminal-GST-tagged H-Ras (1 to 166 residues) binding to NT-647 cysteine-labeled purified SOS1 catalytic domain by competitive microscale thermophoresis analysis	Homo sapiens	4		assay format	Patent Bioactivity Data
3.	ALA4421124	B	Binding affinity to NT-647 cysteine-labeled and N-terminal-GST-tagged recombinant human H-Ras (1 to 166 residues) expressed in Escherichia coli BL21 (DE3) at up to 100 uM by microscale thermophoresis analysis	Homo sapiens	3		assay format	Patent Bioactivity Data
4.	ALA4421140	B	Inhibition of allosteric site activation of N-terminal 6x-His-tagged recombinant human SOS1 catalytic domain (residues 564 to 1049 residues) expressed in Escherichia coli BL21 (DE3) in presence of N-terminal-GST-tagged H-Ras (1 to 166 residues) using BODIPY-fluorescein labeled GDP and GTP by fluorescence-based guanine nucleotide exchange assay	Homo sapiens	3		assay format	Patent Bioactivity Data
5.	ALA4421141	B	Inhibition of N-terminal 6x-His-tagged recombinant human SOS1 catalytic domain (residues 564 to 1049 residues) expressed in Escherichia coli BL21 (DE3) in presence of N-terminal-GST-tagged H-Ras (1 to 166 residues) assessed as inhabitation of catalytic site activation using BODIPY-fluorescein labeled GDP and GTP by fluorescence-based guanine nucleotide exchange assay	Homo sapiens	3		assay format	Patent Bioactivity Data
6.	ALA4421142	B	Binding affinity to N-terminal 6x-His-tagged recombinant human SOS1 catalytic domain (residues 564 to 1049 residues) expressed in Escherichia coli BL21 (DE3) assessed as reduction in N-terminal-GST-tagged H-Ras (1 to 166 residues) binding to NT-647 lysine-labeled purified SOS1 catalytic domain by competitive microscale thermophoresis analysis	Homo sapiens	1		assay format	Patent Bioactivity Data
7.	ALA4421144	B	Inhibition of N-terminal 6x-His-tagged recombinant human SOS1 catalytic domain (residues 564 to 1049 residues) expressed in Escherichia coli BL21 (DE3) assessed as disruption of allosteric SOS1/Ras Y64A mutant:GTPgammaS interaction by Western lot analysis	Homo sapiens	2		assay format	Patent Bioactivity Data