# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4395035 | Binding | Inhibition of human recombinant N-terminal His6-tagged 15-LOX1 expressed in Escherichia coli BL21(DE3) using linoleic acid as substrate preincubated for 8 mins followed by substrate addition by UV absorption analysis | Homo sapiens | 21 | ALA4393683 | assay format | Scientific Literature | |
2. | ALA4395036 | Binding | Inhibition of human recombinant N-terminal His6-tagged 15-LOX1 expressed in Escherichia coli BL21(DE3) using linoleic acid as substrate preincubated for 8 mins followed by substrate addition by UV absorption analysis relative to control | Homo sapiens | 3 | ALA4393683 | assay format | Scientific Literature | |
3. | ALA4395037 | Physicochemical | Stability of compund in pH 7.5 HEPES buffer at 25 uM in presence of human recombinant N-terminal His6-tagged 15-LOX1 expressed in Escherichia coli BL21(DE3) by UV absorption analysis | 8 | ALA4393683 | small-molecule physicochemical format | Scientific Literature | ||
4. | ALA4395038 | Physicochemical | Stability of compund in pH 7.5 HEPES buffer at 25 uM in presence of substrate linoleic acid by UV absorption analysis | 8 | ALA4393683 | small-molecule physicochemical format | Scientific Literature | ||
5. | ALA4395040 | Functional | Antioxidant activity assessed as DPPH radical scavenging activity at 250 uM incubated for 15 mins under dark condition by spectrophotometric method | 2 | ALA4393683 | assay format | Scientific Literature | ||
6. | ALA4395042 | Functional | Antioxidant activity assessed as ferric ion reducing activity by measuring Fe2+ levels using Fe2+-TPTZ measured 15 secs for 5 mins by FRAP assay | 12 | ALA4393683 | assay format | Scientific Literature | ||
7. | ALA4395043 | Binding | Inhibition of human recombinant N-terminal His6-tagged 15-LOX1 expressed in Escherichia coli BL21(DE3) at 0.5 uM using linoleic acid as substrate preincubated for 8 mins followed by substrate addition by UV absorption analysis relative to control | Homo sapiens | 4 | ALA4393683 | assay format | Scientific Literature |