#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4364945	Binding	Inhibition of bovine pancreas alpha-chymotrypsin at 50 uM after 30 mins using N-Succinyl-L-phenylalanine- p-nitroanilide measured for 30 mins	Bos taurus	7	ALA4364253	single protein format	Scientific Literature
2.	ALA4364949	Binding	Binding affinity to Enterovirus 71 3C protease assessed as enzyme-compound covalent adduct formation via Cys147 residue by measuring ratio of peak intensity of covalently modified to unmodified protein at 10 equivalent after 30 mins by HPLC-ESI/MS analysis	Enterovirus A71	3	ALA4364253	assay format	Scientific Literature
3.	ALA4364951	Binding	Irreversible covalent inhibition of Enterovirus 71 3C protease up to 80 uM using NMA-IEALFQGPPK(DNP)FR as substrate by fluorescence method	Enterovirus A71	3	ALA4364253	assay format	Scientific Literature
4.	ALA4364952	Binding	Irreversible covalent inhibition of Enterovirus 71 3C protease assessed as steady-state velocity approaching zero up to 80 uM using NMA-IEALFQGPPK(DNP)FR as substrate by fluorescence method	Enterovirus A71	3	ALA4364253	assay format	Scientific Literature
5.	ALA4364953	Binding	Irreversible covalent inhibition of Enterovirus 71 3C protease assessed as Kinact/Ki ratio using NMA-IEALFQGPPK(DNP)FR as substrate and measured for 1 to 1.5 hrs by fluorescence method	Enterovirus A71	4	ALA4364253	assay format	Scientific Literature
6.	ALA4364954	Functional	Antiviral activity against pseudotyped Enterovirus A71 infected in human muscular rhabdomyosarcoma cells treated prior to viral infection and measured after 24 hrs by luminescence method	Enterovirus A71	32	ALA4364253	organism-based format	Scientific Literature
7.	ALA4364955	ADME	Cytotoxicity against human RD cells at 100 uM after 24 hrs by WST-1 assay relative to control	Homo sapiens	32	ALA4364253	cell-based format	Scientific Literature
8.	ALA4364962	Binding	Reversible inhibition of Enterovirus 71 3C protease assessed as recovery of enzyme activity at 10 to 20 equivalent using peptide substrate pre-incubated for 1 hr followed by dilution prior to substrate addition using ultra-filtered sample relative to control	Enterovirus A71	6	ALA4364253	assay format	Scientific Literature
9.	ALA4364970	Binding	Inhibition of human Cathepsin K at 50 uM using Ac-LR-AFC as substrate preincubated for 10 to 15 mins followed by substrate addition and measured for 30 to 60 mins by fluorescence method relative to control	Homo sapiens	9	ALA4364253	single protein format	Scientific Literature
10.	ALA4364971	Binding	Inhibition of human Calpain1 at 50 uM using Ac-LLY-AFC as substrate preincubated for 5 mins followed by substrate addition and measured after 10 mins by fluorescence method relative to control	Homo sapiens	9	ALA4364253	single protein format	Scientific Literature