# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4371893 | Binding | Inhibition of mouse GAT4 expressed in HEK cells assessed as reduction in [3H]GABA uptake by measuring remaining [3H]GABA uptake levels at 1 uM preincubated for 25 mins followed by [3H]GABA addition and measured after 4 mins by microbeta liquid scintillation counting analysis | Mus musculus | 16 | ALA4371022 | cell-based format | Scientific Literature | |
2. | ALA4371894 | Physicochemical | Hydrolytic stability of compound in Krebs buffer at pH 7.2 at 1 mM measured at 37 degC over 2.5 hrs by 1H-NMR spectroscopy | 2 | ALA4371022 | small-molecule physicochemical format | Scientific Literature | ||
3. | ALA4371896 | Binding | Inhibition of mouse GAT1 expressed in HEK cells assessed as reduction in [3H]GABA uptake at 100 uM by measuring remaining [3H]GABA uptake levels preincubated for 25 mins followed by [3H]GABA addition and measured after 4 mins by microbeta liquid scintillation counting analysis | Mus musculus | 1 | ALA4371022 | cell-based format | Scientific Literature | |
4. | ALA4371898 | Binding | Inhibition of mouse GAT2 expressed in HEK cells assessed as reduction in [3H]GABA uptake at 100 uM by measuring remaining [3H]GABA uptake levels preincubated for 25 mins followed by [3H]GABA addition and measured after 4 mins by microbeta liquid scintillation counting analysis | Mus musculus | 3 | ALA4371022 | cell-based format | Scientific Literature | |
5. | ALA4371899 | Binding | Inhibition of mouse GAT3 expressed in HEK cells assessed as reduction in [3H]GABA uptake by measuring remaining [3H]GABA uptake levels preincubated for 25 mins followed by [3H]GABA addition and measured after 4 mins by microbeta liquid scintillation counting analysis | Mus musculus | 3 | ALA4371022 | cell-based format | Scientific Literature | |
6. | ALA4371901 | Binding | Inhibition of mouse GAT4 expressed in HEK cells assessed as reduction in [3H]GABA uptake by measuring remaining [3H]GABA uptake levels preincubated for 25 mins followed by [3H]GABA addition and measured after 4 mins by microbeta liquid scintillation counting analysis | Mus musculus | 4 | ALA4371022 | cell-based format | Scientific Literature | |
7. | ALA4371902 | Binding | Inhibition of human GAT3 expressed in COS7 cells assessed as reduction in [2H6]GABA uptake preincubated for 25 mins followed by [2H6]GABA addition and measured after 6 mins by LC-MS/MS analysis | Homo sapiens | 4 | ALA4371022 | cell-based format | Scientific Literature |