# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4390491 | B | Inhibition of recombinant human full-length C-terminal FLAG-tagged HDAC1 expressed in baculovirus expression system at 10 nM using Ac-peptide-AMC as substrate preincubated for 15 mins followed by substrate addition by fluorescence assay relative to control | Homo sapiens | 23 | ALA4387776 | assay format | Scientific Literature | |
2. | ALA4390492 | B | Inhibition of recombinant human full-length C-terminal FLAG-tagged HDAC1 expressed in baculovirus expression system at 1000 nM using Ac-peptide-AMC as substrate preincubated for 15 mins followed by substrate addition by fluorescence assay relative to control | Homo sapiens | 23 | ALA4387776 | assay format | Scientific Literature | |
3. | ALA4390493 | B | Inhibition of recombinant human full-length C-terminal FLAG-tagged HDAC1 expressed in baculovirus expression system using Ac-peptide-AMC as substrate preincubated for 15 mins followed by substrate addition by fluorescence assay | Homo sapiens | 23 | ALA4387776 | assay format | Scientific Literature | |
4. | ALA4390494 | B | Inhibition of wild-type human N-terminal GST-tagged CDK2/cycA2 expressed in Sf21 insect cells at 10 nM using FAM-labelled substrate preincubated for 10 mins followed by substrate addition and measured after 30 mins by fluorescence assay relative to control | Homo sapiens | 23 | ALA4387776 | cell-based format | Scientific Literature | |
5. | ALA4390495 | B | Inhibition of wild-type human N-terminal GST-tagged CDK2/cycA2 expressed in Sf21 insect cells at 100 nM using FAM-labelled substrate preincubated for 10 mins followed by substrate addition and measured after 30 mins by fluorescence assay relative to control | Homo sapiens | 23 | ALA4387776 | cell-based format | Scientific Literature | |
6. | ALA4390496 | B | Inhibition of wild-type human N-terminal GST-tagged CDK2/cycA2 expressed in Sf21 insect cells using FAM-labelled substrate preincubated for 10 mins followed by substrate addition and measured after 30 mins by fluorescence assay | Homo sapiens | 23 | ALA4387776 | cell-based format | Scientific Literature | |
7. | ALA4390497 | F | Antiproliferative activity against human A549 cells measured after 72 hrs by CCK8 assay | Homo sapiens | 4 | ALA4387776 | cell-based format | Scientific Literature | |
8. | ALA4390498 | F | Antiproliferative activity against human HepG2 cells measured after 72 hrs by CCK8 assay | Homo sapiens | 4 | ALA4387776 | cell-based format | Scientific Literature | |
9. | ALA4390499 | F | Antiproliferative activity against human CAL148 cells measured after 72 hrs by CCK8 assay | Homo sapiens | 4 | ALA4387776 | cell-based format | Scientific Literature |