# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4433078 | B | Displacement of FITC-Smac from human recombinant His-tagged XIAP BIR3 domain (241 to 356 residues) expressed in Escherichia coli BL21(DE3) cells after 3 hrs by fluorescence polarization assay | Homo sapiens | 7 | cell-based format | Scientific Literature | ||
2. | ALA4433079 | F | Metal chelating activity assessed as compound-Zn2+ complex formation at 50 uM after 15 mins by spectrofluorimetric analysis | 5 | assay format | Scientific Literature | |||
3. | ALA4433080 | F | Metal chelating activity assessed as compound-Zn2+ complex formation at 50 uM by UV-visible spectrophotometric analysis | 1 | assay format | Scientific Literature | |||
4. | ALA4433081 | F | Cytotoxicity against Smac mimetic-sensitive human MDA-MB-231 cells assessed as growth inhibition after 24 hrs by CellTiter-Glo assay | Homo sapiens | 8 | cell-based format | Scientific Literature | ||
5. | ALA4433082 | F | Cytotoxicity against Smac mimetic-resistant human BT549 cells assessed as growth inhibition after 24 hrs by CellTiter-Glo assay | Homo sapiens | 8 | cell-based format | Scientific Literature | ||
6. | ALA4433083 | F | Cytotoxicity against Smac mimetic-resistant human MDA-MB-453 cells assessed as growth inhibition after 24 hrs by CellTiter-Glo assay | Homo sapiens | 8 | cell-based format | Scientific Literature | ||
7. | ALA4433085 | B | Binding affinity to XIAP BIR3 domain in Smac mimetic-sensitive human MDA-MB-231 cells assessed as increase in cellular IAP1 degradation at 5 uM after 24 hrs by Western blot analysis | Homo sapiens | 8 | cell-based format | Scientific Literature | ||
8. | ALA4433086 | B | Binding affinity to XIAP BIR3 domain in Smac mimetic-sensitive human MDA-MB-231 cells assessed as increase in cleaved caspase-3 expression at 5 uM after 24 hrs by Western blot analysis | Homo sapiens | 8 | cell-based format | Scientific Literature | ||
9. | ALA4433087 | B | Binding affinity to XIAP BIR3 domain in Smac mimetic-sensitive human MDA-MB-231 cells assessed as increase in cleaved PARP expression at 5 uM after 24 hrs by Western blot analysis | Homo sapiens | 8 | cell-based format | Scientific Literature | ||
10. | ALA4433088 | F | Effect on XIAP level in Smac mimetic-sensitive human MDA-MB-231 cells at 5 uM after 24 hrs by Western blot analysis | Homo sapiens | 8 | cell-based format | Scientific Literature | ||
11. | ALA4433089 | B | Binding affinity to XIAP BIR3 domain in Smac mimetic-resistant human BT549 cells assessed as increase in cellular IAP1 degradation at 5 uM after 24 hrs by Western blot analysis | Homo sapiens | 3 | assay format | Scientific Literature | ||
12. | ALA4433090 | B | Binding affinity to XIAP BIR3 domain in Smac mimetic-resistant human MDA-MB-453 cells assessed as increase in cellular IAP1 degradation at 5 uM after 24 hrs by Western blot analysis | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
13. | ALA4433092 | B | Binding affinity to XIAP BIR3 domain in Smac mimetic-resistant human MDA-MB-453 cells assessed as increase in cleaved caspase-3 expression at 5 uM after 24 hrs by Western blot analysis | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
14. | ALA4433094 | B | Binding affinity to XIAP BIR3 domain in Smac mimetic-resistant human MDA-MB-453 cells assessed as increase in cleaved PARP expression at 5 uM after 24 hrs by Western blot analysis | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
15. | ALA4433095 | F | Effect on XIAP level in Smac mimetic-resistant human BT549 cells at 5 uM after 24 hrs by Western blot analysis | Homo sapiens | 8 | cell-based format | Scientific Literature | ||
16. | ALA4433096 | F | Effect on XIAP level in Smac mimetic-resistant human MDA-MB-453 cells at 5 uM after 24 hrs by Western blot analysis | Homo sapiens | 8 | cell-based format | Scientific Literature | ||
17. | ALA4433097 | F | Metal chelating activity assessed as Zn2+ chelation by measuring photo-luminescence life time at 0.6 equiv of Zn2+ measured at 10 nanoseconds by time correlated single photon counting method (Rvb = 20%) | 1 | assay format | Scientific Literature | |||
18. | ALA4433098 | F | Metal chelating activity assessed as Zn2+ chelation by measuring photo-luminescence life time at 0.6 equiv of Zn2+ measured at 20 nanoseconds by time correlated single photon counting method (Rvb = 80%) | 1 | assay format | Scientific Literature | |||
19. | ALA4433099 | F | Metal chelating activity assessed as Zn2+ chelation by measuring photo-luminescence life time at 1 equiv of Zn2+ measured at 10 nanoseconds by time correlated single photon counting method (Rvb = 20%) | 1 | assay format | Scientific Literature | |||
20. | ALA4433100 | F | Metal chelating activity assessed as Zn2+ chelation by measuring photo-luminescence life time at 1 equiv of Zn2+ measured at 20 nanoseconds by time correlated single photon counting method (Rvb = 20%) | 1 | assay format | Scientific Literature |