# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4387679 | B | Inhibition of C-terminally His6-tagged human UCK2 expressed in Escherichia coli BL21(DE3) cells incubated for 15 mins before ATP addition and measured after 60 mins by ADP-Glo kinase assay | Homo sapiens | 9 | cell-based format | Scientific Literature | ||
2. | ALA4387680 | B | Inhibition of C-terminally His6-tagged human UCK2 expressed in Escherichia coli BL21(DE3) cells incubated for 15 mins before ATP addition and measured after 60 mins by ADP-Glo kinase assay based HTS assay | Homo sapiens | 7 | cell-based format | Scientific Literature | ||
3. | ALA4387682 | B | Non-competitive inhibition of C-terminally His6-tagged human UCK2 expressed in Escherichia coli BL21(DE3) cells using phosphoenolpyruvate, NADH and varying uridine level by spectrophotometry based pyruvate kinase and lactate dehydrogenase coupled enzyme assay | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
4. | ALA4387683 | B | Non-competitive inhibition of C-terminally His6-tagged human UCK2 expressed in Escherichia coli BL21(DE3) cells using uridine, phosphoenolpyruvate, NADH and varying ATP level by spectrophotometry based pyruvate kinase and lactate dehydrogenase coupled enzyme assay | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
5. | ALA4387684 | B | Inhibition of UCK2 in human K562 cells assessed as inhibition of uridine salvage at 50 uM incubated for 24 hrs by 5-ethynyl-uridine incorporation assay | Homo sapiens | 9 | cell-based format | Scientific Literature |