| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4345394 | B | Inhibition of recombinant human N-terminal TEV cleavage site-fused/FLAG-poly his-tagged TNKS SAM-PARP domain (1024 to 1327 residues) expressed in Escherichia coli assessed as reduction in auto-PARylation preincubated for 10 mins followed by biotinylated-NAD+ addition and measured after 45 mins by ELISA | Homo sapiens | 36 | assay format | Scientific Literature | ||
| 2. | ALA4345395 | B | Inhibition of recombinant human N-terminal TEV cleavage site-fused/FLAG-poly his-tagged TNKS2 ARC5-SAM-PARP domain (613 to 1166 residues) expressed in Escherichia coli assessed as reduction in auto-PARylation preincubated for 10 mins followed by biotinylated-NAD+ addition and measured after 45 mins by ELISA | Homo sapiens | 36 | assay format | Scientific Literature | ||
| 3. | ALA4345396 | B | Inhibition of TNKS/TNKS2 (unknown origin) expressed in HEK293 cells assessed as reduction in Wnt-signaling measured after 24 hrs by TCF-luciferase reporter gene assay | Homo sapiens | 36 | cell-based format | Scientific Literature | ||
| 4. | ALA4345397 | B | Inhibition of TNKS/TNKS2 (unknown origin) expressed in human DLD1 cells assessed as reduction in Wnt-signaling measured after 24 hrs by TCF-luciferase reporter gene assay | Homo sapiens | 36 | cell-based format | Scientific Literature | ||
| 5. | ALA4345398 | F | Antiproliferative activity against human COLO320DM cells assessed as inhibition of cell growth measured after 4 days by CellTiter-Glo assay | Homo sapiens | 36 | cell-based format | Scientific Literature | ||
| 6. | ALA4388120 | A | Cmax in ICR mouse at 50 mg/kg, ip | Mus musculus | 4 | organism-based format | Scientific Literature | ||
| 7. | ALA4388121 | A | Half life in ICR mouse at 50 mg/kg, ip | Mus musculus | 4 | organism-based format | Scientific Literature | ||
| 8. | ALA4388122 | B | Inhibition of recombinant human N-terminal TEV cleavage site-fused/FLAG-poly his-tagged TNKS SAM-PARP domain (1024 to 1327 residues) expressed in Escherichia coli assessed as reduction in auto-PARylation preincubated for 10 mins followed by biotinylated-NAD+ addition and measured after 45 mins by ELISA | Homo sapiens | 57 | assay format | Scientific Literature | ||
| 9. | ALA4388123 | B | Inhibition of recombinant human N-terminal TEV cleavage site-fused/FLAG-poly his-tagged TNKS2 SAM-PARP domain (613 to 1166 residues) expressed in Escherichia coli assessed as reduction in auto-PARylation preincubated for 10 mins followed by biotinylated-NAD+ addition and measured after 45 mins by ELISA | Homo sapiens | 57 | assay format | Scientific Literature | ||
| 10. | ALA4388124 | A | Inhibition of recombinant human PARP1 expressed in Escherichia coli assessed as reduction in auto-PARylation using histone as substrate measured after 45 mins in presence of biotinylated-NAD+ by ELISA | Homo sapiens | 34 | assay format | Scientific Literature | ||
| 11. | ALA4388125 | B | Inhibition of TNKS/TNKS2 (unknown origin) expressed in human DLD1 cells assessed as reduction in Wnt-signaling measured after 24 hrs by TCF-luciferase reporter gene assay | Homo sapiens | 57 | cell-based format | Scientific Literature | ||
| 12. | ALA4388126 | F | Antiproliferative activity against human COLO320DM cells assessed as inhibition of cell growth measured after 4 days by CellTiter-Glo assay | Homo sapiens | 51 | cell-based format | Scientific Literature | ||
| 13. | ALA4388128 | P | Aqueous solubility of the compound in pH 7.4 phosphate buffer at 200 uM incubated for 1 hr under shaking condition by UV spectra analysis | 56 | small-molecule physicochemical format | Scientific Literature | |||
| 14. | ALA4388129 | A | Permeability of the compound by PAMPA | 11 | assay format | Scientific Literature | |||
| 15. | ALA4388130 | A | Protein binding in human serum assessed as unbound fraction at 1 uM measured after 24 hrs by LC-MS/MS analysis | Homo sapiens | 7 | cell-free format | Scientific Literature | ||
| 16. | ALA4388131 | A | Protein binding in mouse serum assessed as unbound fraction at 1 uM measured after 24 hrs by LC-MS/MS analysis | Mus musculus | 7 | cell-free format | Scientific Literature | ||
| 17. | ALA4388132 | A | Stability in human liver microsomes assessed as parent compound remaining at 1 uM measured after 30 mins in presence of NADPH by LC-MS/MS analysis | Homo sapiens | 15 | microsome format | Scientific Literature | ||
| 18. | ALA4388133 | A | Stability in mouse liver microsomes assessed as parent compound remaining at 1 uM measured after 30 mins in presence of NADPH by LC-MS/MS analysis | Mus musculus | 15 | microsome format | Scientific Literature | ||
| 19. | ALA4388134 | F | Antiproliferative activity against human COLO320DM cells assessed inhibition of cell growth measured after 5 days by MTT assay | Homo sapiens | 12 | cell-based format | Scientific Literature | ||
| 20. | ALA4388135 | F | Antiproliferative activity against human RKO cells assessed inhibition of cell growth measured after 5 days by MTT assay | Homo sapiens | 11 | cell-based format | Scientific Literature |
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