| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4358945 | Binding | Inhibition of human N-terminal His6-tagged TEV protease site linked DHPS expressed in Escherichia coli BL21 (DE3) assessed as reduction in radiolabelled amino-butylidene incorporation in eIF5A using [3H]Spermidine trichloride as substrate after 90 to 120 mins in presence of eIF5A and 14 uM NAD+ by Topcount scintillation counting method | Homo sapiens | 21 | ALA4354863 | assay format | Scientific Literature | |
| 2. | ALA4358946 | Binding | Competitive inhibition of human N-terminal His6-tagged TEV protease site linked DHPS expressed in Escherichia coli BL21 (DE3) assessed as reduction in radiolabelled amino-butylidene incorporation in eIF5A using 2 uM of [3H]Spermidine trichloride as substrate after 90 to 120 mins in presence of eIF5A and 250 uM NAD+ by Topcount scintillation counting method | Homo sapiens | 3 | ALA4354863 | assay format | Scientific Literature | |
| 3. | ALA4358947 | Binding | Competitive inhibition of human N-terminal His6-tagged TEV protease site linked DHPS expressed in Escherichia coli BL21 (DE3) assessed as reduction in radiolabelled amino-butylidene incorporation in eIF5A using 100 uM of [3H]Spermidine trichloride as substrate after 90 to 120 mins in presence of eIF5A and 14 uM NAD+ by Topcount scintillation counting method | Homo sapiens | 1 | ALA4354863 | assay format | Scientific Literature | |
| 4. | ALA5247163 | ADME | Metabolic stability in mouse liver microsomes assessed as clearance at 1 uM measured upto 30 mins in the presence of NADPH-generating system by LC-MS analysis | Mus musculus | 9 | ALA5244276 | microsome format | Scientific Literature | |
| 5. | ALA5247164 | Binding | Displacement of [3H]spermidine trihydrochloride from N-terminal 6his tagged TEV fused human DHPS expressed in Escherichia coli BL21 (DE3) incubated for 120 mins in the presence of 14 uM NAD+ by radiometric assay | Homo sapiens | 22 | ALA5244276 | assay format | Scientific Literature | |
| 6. | ALA5247165 | Binding | Displacement of [3H]spermidine trihydrochloride from N-terminal 6his tagged TEV fused human DHPS expressed in Escherichia coli BL21 (DE3) incubated for 120 mins in the presence of 250 uM NAD+ by radiometric assay | Homo sapiens | 9 | ALA5244276 | assay format | Scientific Literature |
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