# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4388122 | Binding | Inhibition of recombinant human N-terminal TEV cleavage site-fused/FLAG-poly his-tagged TNKS SAM-PARP domain (1024 to 1327 residues) expressed in Escherichia coli assessed as reduction in auto-PARylation preincubated for 10 mins followed by biotinylated-NAD+ addition and measured after 45 mins by ELISA | Homo sapiens | 57 | ALA4387706 | assay format | Scientific Literature | |
2. | ALA4388123 | Binding | Inhibition of recombinant human N-terminal TEV cleavage site-fused/FLAG-poly his-tagged TNKS2 SAM-PARP domain (613 to 1166 residues) expressed in Escherichia coli assessed as reduction in auto-PARylation preincubated for 10 mins followed by biotinylated-NAD+ addition and measured after 45 mins by ELISA | Homo sapiens | 57 | ALA4387706 | assay format | Scientific Literature | |
3. | ALA4388124 | ADME | Inhibition of recombinant human PARP1 expressed in Escherichia coli assessed as reduction in auto-PARylation using histone as substrate measured after 45 mins in presence of biotinylated-NAD+ by ELISA | Homo sapiens | 34 | ALA4387706 | assay format | Scientific Literature | |
4. | ALA4388125 | Binding | Inhibition of TNKS/TNKS2 (unknown origin) expressed in human DLD1 cells assessed as reduction in Wnt-signaling measured after 24 hrs by TCF-luciferase reporter gene assay | Homo sapiens | 57 | ALA4387706 | cell-based format | Scientific Literature | |
5. | ALA4388126 | Functional | Antiproliferative activity against human COLO320DM cells assessed as inhibition of cell growth measured after 4 days by CellTiter-Glo assay | Homo sapiens | 51 | ALA4387706 | cell-based format | Scientific Literature | |
6. | ALA4388128 | Physicochemical | Aqueous solubility of the compound in pH 7.4 phosphate buffer at 200 uM incubated for 1 hr under shaking condition by UV spectra analysis | 56 | ALA4387706 | small-molecule physicochemical format | Scientific Literature |