Inhibition of Mycobacterium tuberculosis LeuRS expressed in Escherichia coli Rosetta (DE3) cells at 100 uM incubated for 30 mins followed by incubation with ATP, leucine, tRNA, iPPase for 10 mins and measured after 30 mins by BIOMOL Green reagent based plate reader analysis relative to control
Inhibition of recombinant Mycobacterium tuberculosis H37Rv C-terminal His6-tagged MetRS expressed in Escherichia coli BL21 (DE3) Star cells at 100 uM incubated for 30 mins followed by incubation with ATP, methionine, tRNA, iPPase for 10 mins and measured after 30 mins by BIOMOL Green reagent based plate reader analysis relative to control
Inhibition of Mycobacterium tuberculosis LeuRS expressed in Escherichia coli Rosetta (DE3) cells incubated for 30 mins followed by incubation with ATP, leucine, tRNA, iPPase for 10 mins and measured after 30 mins by BIOMOL Green reagent based plate reader analysis
Inhibition of recombinant Mycobacterium tuberculosis H37Rv C-terminal His6-tagged MetRS expressed in Escherichia coli BL21 (DE3) Star cells incubated for 30 mins followed by incubation with ATP, methionine, tRNA, iPPase for 10 mins and measured after 30 mins by BIOMOL Green reagent based plate reader analysis
Antitubercular activity against Mycobacterium tuberculosis H37Rv ATCC 27294 cultured in medium containing 7H9/glucose/BSA/Tyloxapol assessed as reduction in bacterial growth incubated for 7 days by Alamar blue reagent based assay
Antitubercular activity against Mycobacterium tuberculosis H37Rv ATCC 27294 cultured in medium containing 7H9/glucose/BSA/Tyloxapol assessed as reduction in bacterial growth incubated for 14 days by Alamar blue reagent based assay
Antitubercular activity against Mycobacterium tuberculosis H37Rv ATCC 27294 cultured in medium containing 7H9/DPPC/ cholesterol/BSA/Tyloxapol assessed as reduction in bacterial growth incubated for 7 days by Alamar blue reagent based assay
Antitubercular activity against Mycobacterium tuberculosis H37Rv ATCC 27294 cultured in medium containing 7H9/DPPC/ cholesterol/BSA/Tyloxapol assessed as reduction in bacterial growth incubated for 14 days by Alamar blue reagent based assay
Antitubercular activity against Mycobacterium tuberculosis H37Rv ATCC 27294 cultured in medium containing 7H9/glucose/casitone/Tyloxapol assessed as reduction in bacterial growth incubated for 7 days by Alamar blue reagent based assay
Antitubercular activity against Mycobacterium tuberculosis H37Rv ATCC 27294 cultured in medium containing 7H9/glucose/casitone/Tyloxapol assessed as reduction in bacterial growth incubated for 14 days by Alamar blue reagent based assay
Antitubercular activity against Mycobacterium tuberculosis H37Rv ATCC 27294 cultured in media containing 7H9/DPPC/casitone/Tyloxapol assessed as reduction in bacterial growth incubated for 7 days by Alamar blue reagent based assay
Antitubercular activity against Mycobacterium tuberculosis H37Rv ATCC 27294 cultured in media containing 7H9/DPPC/casitone/Tyloxapol assessed as reduction in bacterial growth incubated for 14 days by Alamar blue reagent based assay