# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4405874 | Binding | Competitive binding affinity to recombinant full length human His-tagged LDH-1 (1 to 322 residues) expressed in Escherichia coli assessed as protein-compound interaction at N-terminal domain after 5 mins by Red-NHS fluorescence dye based microscale thermophoresis analysis | Homo sapiens | 2 | ALA4402594 | assay format | Scientific Literature | |
2. | ALA4405904 | Binding | Binding affinity to truncated human N-terminal His6-tagged LDH-H expressed in Escherichia coli Rosetta (DE3) after 5 mins by Red-NHS fluorescence dye-based microscale thermophoresis analysis | Homo sapiens | 15 | ALA4402594 | assay format | Scientific Literature | |
3. | ALA4405907 | Binding | Competitive binding affinity to human N-terminal His6-tagged LDH-5 expressed in Escherichia coli Rosetta (DE3) assessed as protein-compound interaction at N-terminal domain after 5 mins by Red-NHS fluorescence dye-based microscale thermophoresis analysis | Homo sapiens | 2 | ALA4402594 | assay format | Scientific Literature | |
4. | ALA4405910 | Binding | Binding affinity to recombinant full length human His-tagged LDH-1 (1 to 322 residues) expressed in Escherichia coli assessed as induction of thermal destabilization at 400 uM by nano differential scanning fluorimetry analysis | Homo sapiens | 1 | ALA4402594 | assay format | Scientific Literature | |
5. | ALA4405911 | Binding | Binding affinity to human N-terminal His6-tagged LDH-5 expressed in Escherichia coli Rosetta (DE3) assessed as induction of thermal destabilization at 400 uM by nano differential scanning fluorimetry analysis | Homo sapiens | 1 | ALA4402594 | assay format | Scientific Literature | |
6. | ALA4405912 | Binding | Binding affinity to human N-terminal His6-tagged LDH-5 expressed in Escherichia coli Rosetta (DE3) assessed as induction of thermal destabilization by measuring melting temperature at 400 uM by nano differential scanning fluorimetry analysis (Rvb = 66 degree C) | Homo sapiens | 1 | ALA4402594 | assay format | Scientific Literature | |
7. | ALA4405913 | Binding | Binding affinity to recombinant full length human His-tagged LDH-1 (1 to 322 residues) expressed in Escherichia coli assessed as induction of thermal destabilization by measuring change in melting temperature at 400 uM by nano differential scanning fluorimetry analysis | Homo sapiens | 1 | ALA4402594 | assay format | Scientific Literature | |
8. | ALA4405914 | Binding | Binding affinity to human N-terminal His6-tagged LDH-5 expressed in Escherichia coli Rosetta (DE3) assessed as induction of thermal destabilization by measuring change in melting temperature at 400 uM by nano differential scanning fluorimetry analysis | Homo sapiens | 1 | ALA4402594 | assay format | Scientific Literature | |
9. | ALA4405915 | Binding | Binding affinity to truncated human N-terminal His6-tagged LDH-5 expressed in Escherichia coli Rosetta (DE3) assessed as induction of thermal destabilization by measuring melting temperature at 400 uM by nano differential scanning fluorimetry analysis | Homo sapiens | 1 | ALA4402594 | assay format | Scientific Literature | |
10. | ALA4405918 | Binding | Binding affinity to rabbit LDH-5 assessed as acid-induced dimerization by measuring fluorescence recovery at 0.75 uM to 400 uM pH 7.6 by nano differential scanning fluorimetry analysis | Oryctolagus cuniculus | 1 | ALA4402594 | single protein format | Scientific Literature | |
11. | ALA4405920 | Binding | Binding affinity to human N-terminal His6-tagged human LDH-5 expressed in Escherichia coli Rosetta (DE3) assessed as induction of protein disruption by interacting with tetramerization site by Red-NHS fluorescence dye based microscale thermophoresis analysis | Homo sapiens | 1 | ALA4402594 | assay format | Scientific Literature | |
12. | ALA4405921 | Binding | Binding affinity to truncated N-terminal His6-tagged human LDH-5 expressed in Escherichia coli Rosetta (DE3) assessed as induction of protein disruption by interacting with tetramerization site by Red-NHS fluorescence dye based microscale thermophoresis analysis | Homo sapiens | 1 | ALA4402594 | assay format | Scientific Literature |