#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4309283	Binding	Binding affinity to human RFC expressed in Chinese hamster PC43-10 cells assessed as antiproliferative activity measured as reduction in cell growth after 96 hrs by Cell-Titer Blue assay	Homo sapiens	12	ALA4308835	cell-based format	Scientific Literature
2.	ALA4309284	ADME	Antiproliferative activity in Chinese hamster R2 cells deficient in RFC, PCFT, and FRalpha assessed as reduction in cell growth after 96 hrs by Cell-Titer Blue assay	Cricetulus griseus	12	ALA4308835	cell-based format	Scientific Literature
3.	ALA4309285	Binding	Binding affinity to human FRalpha expressed in Chinese hamster RT16 cells assessed as antiproliferative activity measured as reduction in cell growth after 96 hrs by Cell-Titer Blue assay	Homo sapiens	13	ALA4308835	cell-based format	Scientific Literature
4.	ALA4309286	Binding	Binding affinity to human FRbeta expressed in Chinese hamster D4 cells assessed as antiproliferative activity measured as reduction in cell growth after 96 hrs by Cell-Titer Blue assay	Homo sapiens	13	ALA4308835	cell-based format	Scientific Literature
5.	ALA4309287	Binding	Binding affinity to human PCFT expressed in Chinese hamster R2/PCFT4 cells assessed as antiproliferative activity measured as reduction in cell growth after 96 hrs by Cell-Titer Blue assay	Homo sapiens	13	ALA4308835	cell-based format	Scientific Literature
6.	ALA4309288	Binding	Binding affinity to human RFC2 expressed in human HeLa R1-11 cells assessed as antiproliferative activity measured as reduction in cell growth after 96 hrs by Cell-Titer Blue assay	Homo sapiens	12	ALA4308835	cell-based format	Scientific Literature
7.	ALA4309289	Binding	Binding affinity to human PCFT4 expressed in human HeLa R1-11 cells assessed as antiproliferative activity measured as reduction in cell growth after 96 hrs by Cell-Titer Blue assay	Homo sapiens	12	ALA4308835	cell-based format	Scientific Literature
8.	ALA4309290	Binding	Binding affinity to human FR2 expressed in human HeLa R1-11 cells assessed as antiproliferative activity measured as reduction in cell growth after 96 hrs by Cell-Titer Blue assay	Homo sapiens	12	ALA4308835	cell-based format	Scientific Literature
9.	ALA4309293	Binding	Inhibition of human RFC2 expressed in human HeLa R1-11 cells assessed as reduction in [3H]MTX uptake at pH 7.2 at 10 uM measured over 2 mins relative to control	Homo sapiens	13	ALA4308835	cell-based format	Scientific Literature
10.	ALA4309294	Binding	Inhibition of human PCFT expressed in Chinese hamster R2/PCFT4 cells assessed as reduction in [3H]MTX uptake at pH 5.5 measured over 2 mins by Dixon plot analysis	Homo sapiens	12	ALA4308835	cell-based format	Scientific Literature
11.	ALA4309295	Binding	Inhibition of human PCFT expressed in Chinese hamster R2/PCFT4 cells assessed as reduction in [3H]MTX uptake at pH 5.5 at 1 uM measured over 2 mins relative to control	Homo sapiens	13	ALA4308835	cell-based format	Scientific Literature
12.	ALA4309296	Binding	Inhibition of human PCFT expressed in Chinese hamster R2/PCFT4 cells assessed as reduction in [3H]MTX uptake at pH 5.5 at 10 uM measured over 2 mins relative to control	Homo sapiens	13	ALA4308835	cell-based format	Scientific Literature
13.	ALA4309297	Binding	Inhibition of human PCFT expressed in Chinese hamster R2/PCFT4 cells assessed as reduction in [3H]MTX uptake at pH 5.5 at 1 to 10 uM measured over 2 mins	Homo sapiens	13	ALA4308835	cell-based format	Scientific Literature
14.	ALA4309298	Binding	Displacement of [3H]folic acid from human FRalpha expressed in Chinese hamster RT16 cells at 10 to 1000 nM after 15 mins	Homo sapiens	8	ALA4308835	cell-based format	Scientific Literature
15.	ALA4309299	Binding	Displacement of [3H]folic acid from human FRbeta expressed in Chinese hamster D4 cells at 10 to 1000 nM after 15 mins	Homo sapiens	8	ALA4308835	cell-based format	Scientific Literature
16.	ALA4309300	Binding	Inhibition of GARFTase in human IGROV1 cells assessed as decrease in incorporation of [14C(U)glycine into [14C]formyl GAR formation after 24 hrs	Homo sapiens	12	ALA4308835	cell-based format	Scientific Literature
17.	ALA4309301	Functional	Antiproliferative activity against human IGROV1 cells at 1 to 1000 nM in presence of 320 uM AICA after 96 hrs by Cell-Titer Blue assay	Homo sapiens	4	ALA4308835	cell-based format	Scientific Literature
18.	ALA4309302	Functional	Antiproliferative activity against human IGROV1 cells at 1 to 1000 nM in presence of 10 uM thymidine after 96 hrs by Cell-Titer Blue assay	Homo sapiens	4	ALA4308835	cell-based format	Scientific Literature
19.	ALA4309303	Functional	Antiproliferative activity against human IGROV1 cells at 1 to 1000 nM in presence of 60 uM adenosine after 96 hrs by Cell-Titer Blue assay	Homo sapiens	4	ALA4308835	cell-based format	Scientific Literature
20.	ALA4309307	Functional	Antiproliferative activity against FRalpha knockout human IGROV1 KD10 cells after 96 hrs by Cell-Titer Blue assay	Homo sapiens	12	ALA4308835	cell-based format	Scientific Literature