# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4400458 | B | Inhibition of human recombinant 6His-Q-FLAG-tagged KLK5 expressed in baculovirus infected Sf9 insect cells using (Tos-Gly-Pro-Arg)2[R110].2TFA as substrate measured at 30 secs interval for 5 mins by FLINT assay | Homo sapiens | 28 | ALA4396973 | cell-based format | Scientific Literature | |
2. | ALA4400459 | A | Inhibition of human recombinant N-terminal His-tagged and enterokinase cleavage site-fused KLK1 (19 to 262 residues) expressed in baculovirus infected Sf9 insect cells using R110 labelled GSK3162232A as substrate measured at 30 secs interval for 5 mins by FLINT assay | Homo sapiens | 28 | ALA4396973 | cell-based format | Scientific Literature | |
3. | ALA4400462 | A | Inhibition of recombinant human C-terminal 10-His-tagged factor Xa (L24 to K488 residues) expressed in baculovirus infected Sf21 insect cells using Mca-RPKPVE-Nval-WRK(Dnp)-NH2 as substrate incubated for 40 mins by FLINT assay | Homo sapiens | 15 | ALA4396973 | cell-based format | Scientific Literature | |
4. | ALA4400463 | B | Inhibition of human C-terminal 6-His-tagged KLKB1 (G20-A638 residues) expressed in mouse NS0 cells using PFR-AMC as substrate incubated for 40 mins by FLINT assay | Homo sapiens | 15 | ALA4396973 | cell-based format | Scientific Literature | |
5. | ALA4400464 | B | Inhibition of recombinant human C-terminal 10-His-tagged KLK7 (Glu23 to His252 residues) expressed in mouse NS0 cells using 5-FAM-E-A-L-Y-L-V-S-G-C(5-TAMRA) as substrate incubated for 40 mins by FLINT assay | Homo sapiens | 15 | ALA4396973 | cell-based format | Scientific Literature | |
6. | ALA4400465 | B | Inhibition of recombinant human C-terminal 10-His-tagged KLK8 (Gln29 to Gly260 residues) expressed in mouse NS0 cells using BOC-VPR-AMC as substrate incubated for 40 mins by FLINT assay | Homo sapiens | 15 | ALA4396973 | cell-based format | Scientific Literature | |
7. | ALA4400466 | B | Inhibition of recombinant human C-terminal 10-His-tagged KLK14 (Q19 to M248 residues) expressed in mouse NS0 cells using BOC-VPR-AMC as substrate incubated for 40 mins by FLINT assay | Homo sapiens | 15 | ALA4396973 | cell-based format | Scientific Literature | |
8. | ALA4400467 | A | Inhibition of recombinant human N-terminal Met and 6-His-tagged matriptase (G596 to V855 residues) expressed in Escherichia coli using Boc-QAR-AMC as substrate incubated for 40 mins by FLINT assay | Homo sapiens | 15 | ALA4396973 | assay format | Scientific Literature | |
9. | ALA4400468 | A | Inhibition of human recombinant C-terminal 10-His-tagged thrombin (M1 to E622 residues) expressed in mouse NS0 cells using BOC-VPR-AMC as substrate incubated for 40 mins by FLINT assay | Homo sapiens | 15 | ALA4396973 | cell-based format | Scientific Literature | |
10. | ALA4400469 | A | Inhibition of human neutrophil elastase using MeOSuc-AAPV-AMC as substrate incubated for 40 mins by FLINT assay | Homo sapiens | 15 | ALA4396973 | single protein format | Scientific Literature | |
11. | ALA4400470 | A | Inhibition of recombinant human C-terminal 10-His-tagged urokinase (M1 to L431 residues) expressed in mouse NS0 cells using Z-GGR-AMC as substrate incubated for 40 mins by FLINT assay | Homo sapiens | 15 | ALA4396973 | cell-based format | Scientific Literature | |
12. | ALA4400473 | P | Solubility of the compound in Britton-Robinson buffer at pH 6 measured after 12 hrs | 1 | ALA4396973 | small-molecule physicochemical format | Scientific Literature | ||
13. | ALA4400474 | P | Solubility of the compound in Britton-Robinson buffer at pH 4 measured after 24 hrs | 1 | ALA4396973 | small-molecule physicochemical format | Scientific Literature | ||
14. | ALA4400475 | P | Half life of the compound in Britton-Robinson buffer at pH 4 to 10 at 0.05 mg/ml | 1 | ALA4396973 | small-molecule physicochemical format | Scientific Literature | ||
15. | ALA4400476 | B | Inhibition of human recombinant 6His-Q-FLAG-tagged KLK5 expressed in baculovirus infected Sf9 insect cells using (Tos-Gly-Pro-Arg)2[R110].2TFA as substrate measured at 30 secs interval for 5 mins using 1 x 10'10M enzyme by FLINT assay | Homo sapiens | 15 | ALA4396973 | cell-based format | Scientific Literature | |
16. | ALA4400477 | B | Selectivity ratio of IC50 for human C-terminal 6-His-tagged KLKB1 (G20 to A638 residues) expressed in mouse NS0 cells to IC50 for human recombinant 6His-Q-FLAG-tagged KLK5 expressed in baculovirus infected Sf9 insect cells | Homo sapiens | 15 | ALA4396973 | cell-based format | Scientific Literature | |
17. | ALA4400478 | B | Covalent reversible inhibition of human recombinant 6His-Q-FLAG-tagged KLK5 expressed in baculovirus infected Sf9 insect cells assessed as dissociation half life | Homo sapiens | 2 | ALA4396973 | cell-based format | Scientific Literature | |
18. | ALA4400479 | T | Genotoxicity in Salmonella typhimurium TA1535 in presence of rat S9 fraction by mini-Ames test | Salmonella enterica subsp. enterica serovar Typhimurium | 1 | ALA4396973 | subcellular format | Scientific Literature | |
19. | ALA4400480 | U | Light absorbance of the compound in methanol under UV light irradiation at 290 nm | 1 | ALA4396973 | assay format | Scientific Literature | ||
20. | ALA4400481 | T | Genotoxicity in Salmonella typhimurium TA1537 in presence of rat S9 fraction by mini-Ames test | Salmonella enterica subsp. enterica serovar Typhimurium | 1 | ALA4396973 | subcellular format | Scientific Literature |