# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4315023 | Binding | Inhibition of human NPP1 expressed in COS7 cell membranes at 100 uM using pNP-TMP as substrate preincubated for 3 mins followed by substrate addition and measured after 15 mins relative to control | Homo sapiens | 6 | ALA4311993 | cell-based format | Scientific Literature | |
2. | ALA4315024 | Binding | Inhibition of human NPP3 expressed in COS7 cell membranes at 100 uM using pNP-TMP as substrate preincubated for 3 mins followed by substrate addition and measured after 15 mins relative to control | Homo sapiens | 4 | ALA4311993 | cell-based format | Scientific Literature | |
3. | ALA4315025 | Binding | Inhibition of human NPP1 expressed in COS7 cell membranes using pNP-TMP as substrate preincubated for 3 mins followed by substrate addition and measured after 15 mins by Dixon plot analysis | Homo sapiens | 4 | ALA4311993 | cell-based format | Scientific Literature | |
4. | ALA4315027 | Functional | Agonist activity at human P2Y2 receptor expressed in AD293 cells assessed as inositol-1-phosphate accumulation at 100 uM | Homo sapiens | 7 | ALA4311993 | cell-based format | Scientific Literature | |
5. | ALA4315028 | Functional | Antagonist activity at human P2Y2 receptor expressed in AD293 cells assessed as inhibition of UTP-induced inositol-1-phosphate accumulation at 100 uM | Homo sapiens | 7 | ALA4311993 | cell-based format | Scientific Literature | |
6. | ALA4315030 | Functional | Agonist activity at human P2Y4 receptor expressed in AD293 cells assessed as inositol-1-phosphate accumulation at 0.1 to 100 uM | Homo sapiens | 8 | ALA4311993 | cell-based format | Scientific Literature | |
7. | ALA4315031 | Functional | Antagonist activity at human P2Y4 receptor expressed in AD293 cells assessed as inhibition of UTP-induced inositol-1-phosphate accumulation at 0.1 to 100 uM | Homo sapiens | 8 | ALA4311993 | cell-based format | Scientific Literature | |
8. | ALA4315032 | Functional | Antagonist activity at human P2Y6 receptor expressed in AD293 cells assessed as inhibition of UDP-induced inositol-1-phosphate accumulation at 0.1 to 100 uM | Homo sapiens | 8 | ALA4311993 | cell-based format | Scientific Literature | |
9. | ALA4315033 | Functional | Agonist activity at human P2Y6 receptor expressed in AD293 cells assessed as inositol-1-phosphate accumulation at 100 uM | Homo sapiens | 5 | ALA4311993 | cell-based format | Scientific Literature | |
10. | ALA4315035 | Binding | Inhibition of alkaline phosphatase in human primary chondrocyte at 100 uM using p-nitrophenyl phosphate as substrate after 30 mins by ELISA relative to control | Homo sapiens | 4 | ALA4311993 | assay format | Scientific Literature | |
11. | ALA4315036 | ADME | Cytotoxicity against human primary chondrocytes at 100 uM after 24 hrs by XTT assay | Homo sapiens | 4 | ALA4311993 | assay format | Scientific Literature |