#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4386909	Binding	Inhibition of Trypanosoma cruzi cruzain assessed as reduction in 7-amino-4-methylcoumarin production using Z-FR-AMC as substrate and measured up to 60 mins by fluorescence based method	Trypanosoma cruzi	3	ALA4385629	single protein format	Scientific Literature
2.	ALA4386910	Binding	Activation of Trypanosoma cruzi cruzain assessed as increase in enzyme activity using Z-FR-AMC as substrate at 0.1 mM incubated for 15 mins in dark and measured at 5 mins interval for 60 mins by fluorescence based method relative to control	Trypanosoma cruzi	3	ALA4385629	single protein format	Scientific Literature
3.	ALA4767553	Binding	Inhibition of wild type recombinant HIV-1 subtype B BH10 p66/p51 reverse transcriptase heterodimer expressed in Escherichia coli BL21 (DE3) assessed as inhibition of dTTP incorporation on poly(rA)-oligo(dT) template primer duplex at 10 uM preincubated with enzyme for 2 mins followed by incubation with poly(A)-oligo(dT) templates for 2 hrs by flourescence assay method	Human immunodeficiency virus type 1 BH10	26	ALA4765246	assay format	Scientific Literature
4.	ALA4767554	Binding	Inhibition of wild type recombinant HIV-1 subtype B BH10 p66/p51 reverse transcriptase heterodimer expressed in Escherichia coli BL21 (DE3) assessed as inhibition of dTTP incorporation on poly(rA)-oligo(dT) template primer duplex at 100 uM preincubated with enzyme for 2 mins followed by incubation with poly(A)-oligo(dT) templates for 2 hrs by flourescence assay method	Human immunodeficiency virus type 1 BH10	26	ALA4765246	assay format	Scientific Literature