#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4364946	Binding	Binding affinity to Enterovirus 71 3C protease assessed as enzyme-compound covalent adduct formation via Cys147 residue by measuring ratio of peak intensity of covalently modified to unmodified protein at 20 equivalent after 1 hr by HPLC-ESI/MS analysis	Enterovirus A71	1	ALA4364253	assay format	Scientific Literature
2.	ALA4364949	Binding	Binding affinity to Enterovirus 71 3C protease assessed as enzyme-compound covalent adduct formation via Cys147 residue by measuring ratio of peak intensity of covalently modified to unmodified protein at 10 equivalent after 30 mins by HPLC-ESI/MS analysis	Enterovirus A71	3	ALA4364253	assay format	Scientific Literature
3.	ALA4364953	Binding	Irreversible covalent inhibition of Enterovirus 71 3C protease assessed as Kinact/Ki ratio using NMA-IEALFQGPPK(DNP)FR as substrate and measured for 1 to 1.5 hrs by fluorescence method	Enterovirus A71	4	ALA4364253	assay format	Scientific Literature
4.	ALA4364954	Functional	Antiviral activity against pseudotyped Enterovirus A71 infected in human muscular rhabdomyosarcoma cells treated prior to viral infection and measured after 24 hrs by luminescence method	Enterovirus A71	32	ALA4364253	organism-based format	Scientific Literature
5.	ALA4364955	ADME	Cytotoxicity against human RD cells at 100 uM after 24 hrs by WST-1 assay relative to control	Homo sapiens	32	ALA4364253	cell-based format	Scientific Literature
6.	ALA4364956	Binding	Irreversible covalent inhibition of Enterovirus 71 3C protease at 64 uM using NMA-IEALFQGPPK(DNP)FR as substrate and measured for 1 to 1.5 hrs by fluorescence method	Enterovirus A71	1	ALA4364253	assay format	Scientific Literature