# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4479999 | Binding | Inhibition of Bacillus anthracis Sterne His-tagged DHOase expressed in Escherichia coli BL21(DE3) Gold cells using DHO as substrate assessed as enzyme activity at 25 or 400 uM preincubated for 10 mins followed by substrate addition measured after 30 mins by DAMO-TSC reagent based colorimetric assay relative to control | Bacillus anthracis str. Sterne | 8 | ALA4477258 | cell-based format | Scientific Literature | |
2. | ALA4480000 | Binding | Binding affinity to Bacillus anthracis Sterne His-tagged DHOase expressed in Escherichia coli BL21(DE3) Gold cells assessed as change in melting temperature at 400 uM incubated for 10 mins by SYPRO orange dye based fluorescence thermal shift assay | Bacillus anthracis str. Sterne | 8 | ALA4477258 | cell-based format | Scientific Literature | |
3. | ALA4480001 | Binding | Binding affinity to Bacillus anthracis Sterne His-tagged DHOase expressed in Escherichia coli BL21(DE3) Gold cells by surface plasmon resonance analysis | Bacillus anthracis str. Sterne | 12 | ALA4477258 | cell-based format | Scientific Literature | |
4. | ALA4480002 | Binding | Binding affinity to Bacillus anthracis Sterne His-tagged DHOase expressed in Escherichia coli BL21(DE3) Gold cells in presence of N-carbamyl-L-aspartate by surface plasmon resonance analysis | Bacillus anthracis str. Sterne | 12 | ALA4477258 | cell-based format | Scientific Literature |