# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4399640 | A | Cytotoxicity against mouse RAW264.7 cells assessed as reduction in cell viability at 30 to 60 uM incubated for 24 hrs by MTT assay | Mus musculus | 4 | ALA4396930 | cell-based format | Scientific Literature | |
2. | ALA4399641 | B | Displacement of 1,8-ANS from human recombinant full length N-terminal his-tagged FABP4 expressed in Escherichia coli BL21 (DE3) at 25 uM incubated for 3 mins by fluorescence based assay relative to control | Homo sapiens | 37 | ALA4396930 | assay format | Scientific Literature | |
3. | ALA4399642 | B | Displacement of 1,8-ANS from human recombinant full length N-terminal his-tagged FABP5 expressed in Escherichia coli BL21 (DE3) at 25 uM incubated for 3 mins by fluorescence based assay relative to control | Homo sapiens | 35 | ALA4396930 | assay format | Scientific Literature | |
4. | ALA4399643 | B | Displacement of 1,8-ANS from human recombinant full length N-terminal his-tagged FABP4 expressed in Escherichia coli BL21 (DE3) incubated for 3 mins by fluorescence based assay | Homo sapiens | 26 | ALA4396930 | assay format | Scientific Literature | |
5. | ALA4399644 | B | Displacement of 1,8-ANS from human recombinant full length N-terminal his-tagged FABP5 expressed in Escherichia coli BL21 (DE3) incubated for 3 mins by fluorescence based assay | Homo sapiens | 23 | ALA4396930 | assay format | Scientific Literature | |
6. | ALA4399645 | A | Displacement of 1,8-ANS from FABP3 (unknown origin) incubated for 3 mins by fluorescence based assay | Homo sapiens | 3 | ALA4396930 | single protein format | Scientific Literature | |
7. | ALA4399646 | B | Selectivity ratio of IC50 for FABP3 (unknown origin) to IC50 for human recombinant full length N-terminal his-tagged FABP4 expressed in Escherichia coli BL21 (DE3) | Homo sapiens | 3 | ALA4396930 | assay format | Scientific Literature | |
8. | ALA4399647 | B | Selectivity ratio of IC50 for FABP3 (unknown origin) to IC50 for human recombinant full length N-terminal his-tagged FABP5 expressed in Escherichia coli BL21 (DE3) | Homo sapiens | 3 | ALA4396930 | assay format | Scientific Literature | |
9. | ALA4399648 | A | Cytotoxicity against mouse 3T3L1 cells assessed as reduction in cell viability at 30 to 60 uM incubated for 24 hrs by MTT assay | Mus musculus | 4 | ALA4396930 | cell-based format | Scientific Literature | |
10. | ALA4399649 | B | Inhibition of FABP4 in mouse 3T3L1 cells assessed as inhibition of forskolin-stimulated lipolysis at 30 uM preincubated for 24 hrs followed by forskolin addition and measured after 2 hrs | Mus musculus | 4 | ALA4396930 | cell-based format | Scientific Literature | |
11. | ALA4399655 | B | Inhibition of FABP4 in mouse RAW264.7 cells assessed as decrease in LPS-induced NF-kappaB phosphorylation at 30 to 60 uM preincubated for 12 hrs followed by LPS addition and measured after 10 mins by Western blot analysis | Mus musculus | 4 | ALA4396930 | cell-based format | Scientific Literature | |
12. | ALA4399656 | B | Inhibition of FABP4 in mouse RAW264.7 cells assessed as decrease in LPS-induced IKK phosphorylation at 30 to 60 uM preincubated for 12 hrs followed by LPS addition and measured after 10 mins by Western blot analysis | Mus musculus | 4 | ALA4396930 | cell-based format | Scientific Literature | |
13. | ALA5262841 | B | Binding affinity to human FABP3 expressed in Escherichia coli assessed as degree of fluorescent shift by temperature-dependent fluorescence thermal shift assay | Homo sapiens | 4 | ALA5260812 | assay format | Scientific Literature | |
14. | ALA5262842 | B | Binding affinity to human FABP4 expressed in Escherichia coli assessed as degree of fluorescent shift by temperature-dependent fluorescence thermal shift assay | Homo sapiens | 4 | ALA5260812 | assay format | Scientific Literature | |
15. | ALA5262843 | B | Displacement of BODIPY 500/510 C4 from human FABP3 expressed in Escherichia coli incubated for 30 mins by FP assay | Homo sapiens | 4 | ALA5260812 | assay format | Scientific Literature | |
16. | ALA5262844 | B | Displacement of BODIPY 500/510 C4 from human FABP4 expressed in Escherichia coli incubated for 30 mins by FP assay | Homo sapiens | 4 | ALA5260812 | assay format | Scientific Literature |