# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4308556 | Binding | Inhibition of human recombinant 5-LOX expressed in Escherichia coli BL21 assessed as conversion of arachidonic acid to 5-HPETE at 10 uM using arachidonic acid as substrate in presence of ATP by UV-vis spectrophotometry analysis relative to control | Homo sapiens | 23 | ALA4304831 | assay format | Scientific Literature | |
2. | ALA4308557 | Binding | Inhibition of human recombinant 5-LOX expressed in Escherichia coli BL21 assessed as conversion of arachidonic acid to 5-HPETE using arachidonic acid as substrate in presence of ATP by UV-vis spectrophotometry analysis | Homo sapiens | 9 | ALA4304831 | assay format | Scientific Literature | |
3. | ALA4308558 | Functional | Antioxidant activity assessed as DPPH radical scavenging activity at 10 uM after 30 mins under dark condition relative to control | 24 | ALA4304831 | assay format | Scientific Literature | ||
4. | ALA4308559 | Binding | Inhibition of human recombinant 5-LOX pseudoperoxidase activity expressed in Escherichia coli BL21 assessed as compound redox activity by measuring change in absorption at 10 uM using 13(S)-HpODE as substrate in presence of ATP by UV-vis spectrophotometric method relative to control | Homo sapiens | 9 | ALA4304831 | assay format | Scientific Literature | |
5. | ALA4308564 | Binding | Competitive inhibition of human recombinant 5-LOX expressed in Escherichia coli BL21 assessed as Vmax for substrate at 2 uM using varying levels arachidonic acid as substrate by Lineweaver-Burk plot analysis (Rvb = 0.33 to 0.45 +/- 0.01 nmol/min) | Homo sapiens | 2 | ALA4304831 | assay format | Scientific Literature | |
6. | ALA4308565 | Binding | Competitive inhibition of human recombinant 5-LOX expressed in Escherichia coli BL21 assessed as Vmax for substrate at 5 uM using varying levels arachidonic acid as substrate by Lineweaver-Burk plot analysis (Rvb = 0.33 to 0.45 +/- 0.01 nmol/min) | Homo sapiens | 2 | ALA4304831 | assay format | Scientific Literature | |
7. | ALA4308566 | Binding | Competitive inhibition of human recombinant 5-LOX expressed in Escherichia coli BL21 assessed as increase in Km for substrate at 2 uM using varying levels arachidonic acid as substrate by Lineweaver-Burk plot analysis (Rvb = 0.31 to 0.35 +/- 0.08 to 0.10 uM) | Homo sapiens | 2 | ALA4304831 | assay format | Scientific Literature | |
8. | ALA4308567 | Binding | Competitive inhibition of human recombinant 5-LOX expressed in Escherichia coli BL21 assessed as increase in Km for substrate at 5 uM using varying levels arachidonic acid as substrate by Lineweaver-Burk plot analysis (Rvb = 0.31 to 0.35 +/- 0.08 to 0.10 uM) | Homo sapiens | 2 | ALA4304831 | assay format | Scientific Literature |