#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4428139	B	Activation of full-length GST-tagged recombinant human SIRT1 assessed as enzyme activity obtained without subtraction of intrinsic fluorescence using H3K9 as substrate at 50 uM after 30 mins by SIRTainty assay	Homo sapiens	56	ALA4428023	single protein format	Scientific Literature
2.	ALA4428140	B	Activation of full-length GST-tagged recombinant human SIRT1 assessed as enzyme activity obtained with subtraction of intrinsic fluorescence using H3K9 as substrate at 50 uM after 30 mins by SIRTainty assay	Homo sapiens	51	ALA4428023	single protein format	Scientific Literature
3.	ALA4428141	B	Inhibition of recombinant human PDE4D assessed as phosphate release using cAMP as substrate at 50 uM after 60 mins by malachite green assay	Homo sapiens	6	ALA4428023	single protein format	Scientific Literature
4.	ALA4428142	B	Activation of AMPK/SIRT1 in human HaCaT cells assessed as nitric oxide production at 1 uM incubated for 1 hr by DAF-2D staining-based fluorescence assay	Homo sapiens	7	ALA4428023	cell-based format	Scientific Literature
5.	ALA4428143	B	Activation of AMPK/SIRT1 in human HaCaT cells assessed as nitric oxide production incubated for 1 hr by DAF-2D staining-based fluorescence assay	Homo sapiens	3	ALA4428023	cell-based format	Scientific Literature
6.	ALA4428144	B	Activation of AMPK/SIRT1 in human HaCaT cells assessed as nitric oxide production at 10 uM incubated for 1 hr in presence of compound C by DAF-2D staining-based fluorescence assay	Homo sapiens	3	ALA4428023	cell-based format	Scientific Literature
7.	ALA4428145	B	Activation of AMPK/SIRT1 in human HaCaT cells assessed as nitric oxide production at 10 uM incubated for 1 hr in presence of EX-527 by DAF-2D staining-based fluorescence assay	Homo sapiens	3	ALA4428023	cell-based format	Scientific Literature
8.	ALA4428146	B	Activation of SIRT1 in human HaCaT cell nuclear extract at 1 uM by fluorimetric assay	Homo sapiens	4	ALA4428023	cell-based format	Scientific Literature
9.	ALA4428148	B	Activation of SIRT1 in human HaCaT cells assessed as H4K16 deacetylation at 1 uM after 1 hr by Western blotting method	Homo sapiens	5	ALA4428023	cell-based format	Scientific Literature
10.	ALA4428149	F	Wound healing activity in CD1 mouse assessed as decrease in epithelial gap at 1 uM administered topically on wound everyday for 14 days by biopsy punch assay	Mus musculus	3	ALA4428023	organism-based format	Scientific Literature
11.	ALA4428150	F	Wound healing activity in CD1 mouse assessed as increased wound closure at 1 uM administered topically on wound everyday for 14 days by biopsy punch assay	Mus musculus	3	ALA4428023	organism-based format	Scientific Literature
12.	ALA4428151	B	Activation of AMPK/SIRT1 in mouse C2C12 cells assessed as increase in mitochondrial density at 30 uM after 16 hrs by Mitotracker Green staining-based confocal microscopy	Mus musculus	3	ALA4428023	cell-based format	Scientific Literature
13.	ALA4428152	B	Activation of AMPK/SIRT1 in mouse C2C12 cells assessed as increase in mitochondrial density at 30 uM after 16 hrs by Mitotracker Green staining-based confocal microscopy in presence of compound C	Mus musculus	2	ALA4428023	cell-based format	Scientific Literature
14.	ALA4428153	B	Activation of AMPK/SIRT1 in mouse C2C12 cells assessed as increase in mitochondrial density at 30 uM after 16 hrs by Mitotracker Green staining-based confocal microscopy in presence of EX-527	Mus musculus	2	ALA4428023	cell-based format	Scientific Literature
15.	ALA4428154	B	Antagonist activity at calcium channel in guinea pig smooth muscle assessed as inhibition of calcium-induced contraction in K+-depolarized aortic strips at 50 uM	Cavia porcellus	2	ALA4428023	assay format	Scientific Literature
16.	ALA4428157	F	Antiproliferative activity against human M14 cells assessed as cell viability after 72 hrs by MTT assay	Homo sapiens	2	ALA4428023	cell-based format	Scientific Literature
17.	ALA4428158	F	Antiproliferative activity against human SAN cells assessed as cell viability after 72 hrs by MTT assay	Homo sapiens	2	ALA4428023	cell-based format	Scientific Literature
18.	ALA4428159	F	Antiproliferative activity against human A549 cells assessed as cell viability after 72 hrs by MTT assay	Homo sapiens	2	ALA4428023	cell-based format	Scientific Literature
19.	ALA4428160	F	Antiproliferative activity against human NCI-H1299 cells assessed as cell viability after 72 hrs by MTT assay	Homo sapiens	2	ALA4428023	cell-based format	Scientific Literature
20.	ALA4428161	F	Antiproliferative activity against human HT-29 cells assessed as cell viability after 72 hrs by MTT assay	Homo sapiens	2	ALA4428023	cell-based format	Scientific Literature