# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4411820 | Binding | Substrate activity at recombinant human NQO1 assessed as ROS generation by measuring metabolic rate utilizing umol of NADPH per umol of enzyme at 10 uM preincubated for 3 mins followed by NADPH addition and measured at 2 secs interval for 5 mins | Homo sapiens | 33 | ALA4411270 | single protein format | Scientific Literature | |
2. | ALA4411821 | Binding | Substrate activity at recombinant human NQO1 assessed as ratio of Kcat to Km by measuring umol of NADPH utilized per umol of enzyme at 10 uM preincubated for 3 mins followed by NADPH addition and measured at 2 secs interval for 5 mins by Michaelis-Menten plot analysis | Homo sapiens | 4 | ALA4411270 | single protein format | Scientific Literature | |
3. | ALA4411822 | Functional | Cytotoxicity against human A549/TR cells expressing NQO1 assessed as reduction in cell viability after 72 hrs by MTT assay | Homo sapiens | 17 | ALA4411270 | cell-based format | Scientific Literature | |
4. | ALA4411823 | Functional | Cytotoxicity against human A549 cells expressing NQO1 assessed as reduction in cell viability after 72 hrs by MTT assay | Homo sapiens | 17 | ALA4411270 | cell-based format | Scientific Literature | |
5. | ALA4411824 | ADME | Cytotoxicity against NQO1 deficient human L02 cells assessed as reduction in cell viability after 72 hrs by MTT assay | Homo sapiens | 17 | ALA4411270 | cell-based format | Scientific Literature | |
6. | ALA4411825 | Toxicity | Selectivity ratio of IC50 for NQO1 deficient human L02 cells to IC50 for human A549/TR cells expressing NQO1 | Homo sapiens | 17 | ALA4411270 | assay format | Scientific Literature | |
7. | ALA4411836 | Binding | Substrate activity at recombinant human NQO1 assessed as ROS generation by measuring umol of cytochrome c utilized per umol of enzyme at 10 uM measured at 2 secs interval for 5 mins in presence of NADPH | Homo sapiens | 6 | ALA4411270 | single protein format | Scientific Literature | |
8. | ALA4411912 | Binding | Substrate activity at recombinant human NQO1 assessed as ratio of number of equivalents of consumed NADPH to number of equivalents of test compound at 10 uM preincubated for 3 mins followed by NADPH addition and measured at 2 secs interval for 5 mins by Michaelis-Menten plot analysis | Homo sapiens | 30 | ALA4411270 | single protein format | Scientific Literature | |
9. | ALA5234060 | Binding | Inhibition of human USP7 using Ub-AMC as substrate | Homo sapiens | 42 | ALA5233229 | single protein format | Scientific Literature | |
10. | ALA5234065 | Binding | Inhibition of USP47 (unknown origin) | Homo sapiens | 21 | ALA5233229 | single protein format | Scientific Literature | |
11. | ALA5234075 | Binding | Inhibition of human USP8 using Ub-AMC as substrate | Homo sapiens | 24 | ALA5233229 | single protein format | Scientific Literature | |
12. | ALA5234076 | Binding | Inhibition of USP8 (unknown origin) | Homo sapiens | 11 | ALA5233229 | single protein format | Scientific Literature |