# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4325325 | Binding | Inhibition of N-terminal GST-fused full-length human CDC7 (1 to 574 end residues)/DBF4 (1 to 674 residues) expressed in baculovirus expression system using MCM2 as substrate preincubated with enzyme for 10 mins prior to 1 uM ATP addition by HTRF transcreener ADP assay | Homo sapiens | 17 | ALA4321874 | assay format | Scientific Literature | |
2. | ALA4325326 | Binding | Inhibition of N-terminal GST-tagged full-length human CDK2 (1 to 298 residues)/Cyclin E (1 to 410 residues) expressed in baculovirus expression system using histone H1 as susbtrate after 90 mins by Kinase-Glo luminescence assay | Homo sapiens | 17 | ALA4321874 | assay format | Scientific Literature | |
3. | ALA4325327 | Binding | Inhibition of N-terminal GST-tagged human ROCK1 catalytic domain (1 to 477 residues) expressed in baculovirus expression system using biotin-STK-substrate 2 as substrate preincubated with enzyme for 5 mins followed by ATP addition and measured after 2 hrs by TR-FRET assay | Homo sapiens | 17 | ALA4321874 | assay format | Scientific Literature | |
4. | ALA4325328 | Binding | Selectivity index, ratio of IC50 for N-terminal GST-tagged full-length human CDK2 (1 to 298 residues)/Cyclin E (1 to 410 residues) expressed in baculovirus expression system to IC50 for N-terminal GST-fused full-length human CDC7 (1 to 574 end residues)/DBF4 (1 to 674 residues) expressed in baculovirus expression system | Homo sapiens | 17 | ALA4321874 | assay format | Scientific Literature | |
5. | ALA4325329 | Binding | Selectivity index, ratio of IC50 N-terminal GST-tagged human ROCK1 catalytic domain (1 to 477 residues) expressed in baculovirus expression system to IC50 for N-terminal GST-fused full-length human CDC7 (1 to 574 end residues)/DBF4 (1 to 674 residues) expressed in baculovirus expression system | Homo sapiens | 17 | ALA4321874 | assay format | Scientific Literature | |
6. | ALA4325330 | Binding | Inhibition of N-terminal GST-fused full-length human CDC7 (1 to 574 end residues)/DBF4 (1 to 674 residues) expressed in baculovirus expression system using MCM2 as substrate in presence of 50 uM ATP by HTRF transcreener ADP assay | Homo sapiens | 5 | ALA4321874 | assay format | Scientific Literature | |
7. | ALA4325331 | Binding | Inhibition of N-terminal GST-fused full-length human CDC7 (1 to 574 end residues)/DBF4 (1 to 674 residues) expressed in baculovirus expression system using MCM2 as substrate preincubated with enzyme for 60 mins prior to 50 uM ATP addition by HTRF transcreener ADP assay | Homo sapiens | 5 | ALA4321874 | assay format | Scientific Literature | |
8. | ALA4325332 | Binding | Inhibition of CDC7 in human HeLa cells assessed as inhibition of MCM2 phosphorylation at Ser40 residue in human HeLa cells after 7 hrs by Western blot analysis | Homo sapiens | 5 | ALA4321874 | cell-based format | Scientific Literature | |
9. | ALA4325333 | Functional | Antiproliferative activity against human COLO205 cells assessed as reduction in cell growth after 3 days by CellTiter-Glo luminescence assay | Homo sapiens | 5 | ALA4321874 | cell-based format | Scientific Literature | |
10. | ALA4325363 | Binding | Inhibition of ATP-specific reporter probe binding to CDC7 (unknown origin) assessed as equilibrium dissociation constant incubated for 60 mins by proteros reporter displacement assay | Homo sapiens | 4 | ALA4321874 | single protein format | Scientific Literature | |
11. | ALA4325364 | Binding | Inhibition of ATP-specific reporter probe binding to CDC7 (unknown origin) assessed as dissociation rate constant incubated for 60 mins by proteros reporter displacement assay | Homo sapiens | 4 | ALA4321874 | single protein format | Scientific Literature | |
12. | ALA4325365 | Binding | Inhibition of ATP-specific reporter probe binding to CDC7 (unknown origin) assessed as residence time incubated for 60 mins by proteros reporter displacement assay | Homo sapiens | 4 | ALA4321874 | single protein format | Scientific Literature |