# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4323086 | Binding | Binding affinity to recombinant human His-tagged SPIN1 (26 to 262 residues) expressed in Escherichia coli BL21 (DE3) assessed as melting temperature at 20 uM by Sypro orange dye based differential scanning fluorimetry | Homo sapiens | 6 | ALA4321817 | assay format | Scientific Literature | |
2. | ALA4323097 | Binding | Displacement of trimethylated biotinylated histone H3 peptide from recombinant human His-tagged SPIN1 (26 to 262 residues) expressed in Escherichia coli BL21 (DE3) incubated for 30 mins followed by peptide addition and measured after 1 hr by Alphascreen method | Homo sapiens | 18 | ALA4321817 | assay format | Scientific Literature | |
3. | ALA4323101 | Binding | Binding affinity to human His-tagged SPIN1 (21 to 262 residues) expressed in Escherichia coli BL21 (DE3) assessed as dissociation constant preincubated for 5 mins followed by compound addition by octet BLI assay | Homo sapiens | 6 | ALA4321817 | assay format | Scientific Literature | |
4. | ALA4323102 | Binding | Binding affinity to recombinant human His-tagged SPIN1 (49 to 262 residues) expressed in Escherichia coli BL21 (DE3) assessed as dissociation constant by isothermal titration calorimetry | Homo sapiens | 7 | ALA4321817 | assay format | Scientific Literature |